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Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens

https://doi.org/10.21055/0370-1069-2012-2(112)-65-68

Abstract

Studied was dissemination of avian influenza virus (AIV) in the organism of chickens after intranasal challenge with 10-100 LD50. The primary organ of accumulation of AIV A/H5N1 (A/Chicken/Kurgan/05/2005strain) is the respiratory tract (nasal mucosa), where the virus is registered in 18 hours after challenge. The accumulation of pathogen is observed in many organs and serum of chicken in 30-32 hours after challenge. The animals die in 54 hours, the concentration of virus reaches critical value in all studied samples. The highest AIV loads (7 lg of chicken embryo infective dose - EID50/g or ml) are registered in lungs, blood serum and kidneys of chicken. The results of AIV loads measuring using titration and real time RT-PCR show high degree of correlation (r=0.89).

About the Authors

O. K. Demina
State Research Centre of Virology and Biotechnology Vector
Russian Federation


Ar. A. Sergeev
State Research Centre of Virology and Biotechnology Vector
Russian Federation


O. V. P'yankov
State Research Centre of Virology and Biotechnology Vector
Russian Federation


A. N. Shikov
State Research Centre of Virology and Biotechnology Vector
Russian Federation


Al. A. Sergeev
State Research Centre of Virology and Biotechnology Vector
Russian Federation


S. A. Berillo
State Research Centre of Virology and Biotechnology Vector
Russian Federation


E. I. Sergeeva
State Research Centre of Virology and Biotechnology Vector
Russian Federation


A. P. Agafonov
State Research Centre of Virology and Biotechnology Vector
Russian Federation


A. N. Sergeev
State Research Centre of Virology and Biotechnology Vector
Russian Federation


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Review

For citations:


Demina O.K., Sergeev A.A., P'yankov O.V., Shikov A.N., Sergeev A.A., Berillo S.A., Sergeeva E.I., Agafonov A.P., Sergeev A.N. Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens. Problems of Particularly Dangerous Infections. 2012;(2(112)):65-68. (In Russ.) https://doi.org/10.21055/0370-1069-2012-2(112)-65-68

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ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)