Peculiarities of Antibody Genesis in the Laboratory Animals Immunized with Anthrax Protective Antigen Preparation, Bacillus anthracis Recombinant or Bacillus anthracis Vaccine Strains
https://doi.org/10.21055/0370-1069-2012-2(112)-75-77
Abstract
Protective antigen, extracted from Bacillus anthracis asporogenic recombinant strain, triggers progression of a high-grade adaptive immunity in vivo. Two-fold immunization of rabbits with the purified protein preparation in a dose of 50 mcg induces production of specific antibodies in titers higher than those registered in case of immunization with vaccine or genetically engineered anthrax strains. It is determined that the recombinant B. anthracis strain initiates further production of antibodies towards S-layer protein of anthrax agent.
About the Authors
P. Yu. Popova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. I. Mikshis
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
A. Yu. Goncharova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
T. N. Kashtanova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
Yu. A. Popov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
References
1. Marinin L.I., Onishchenko G.G., Kravchenko T.B., Dyatlov I.A., Tyurin E.A., Stepanov A.V., Nikiforov V.V. [Anthrax in Humans: Epidemiology, Prophylaxis, Diagnostics, and Treatment]. M.: ZAO MP “Gigiena”; 2008. 416 p.
2. Mikshis N.I., Kudryavtseva O.M., Bolotnikova M.F., Shulepov D.V., Novikova L.V., Popov Yu.A. et al. [Immunogenicity of the recombinant bacil- lary strains with the genetically engineered gene responsible for Bacillus an- thracis protective antigen synthesis]. Mol. Genet. Mikrobiol. Virusol. 2007; 3:15–21.
3. Mikshis N.I., Kudryavtseva O.M., Shulepov D.V., Goncharova A.Yu., Bolotnikova M.F., Novikova L.V. et al. [Asporogenic recombinant producer of anthrax agent protective antigen]. Biotekhnologia 2010; 4:25–33.
4. Brossier F., Levy M., Mock M. Anthrax spores make an essential contribution to vaccine efficacy. Infect. Immun. 2002; 70:661-4.
5. Chitlaru T., Altboum Z., Reuveny S., Shafferman A. Progress and novel strategies in vaccine development and treatment of anthrax. Immunol. Rev. 2011; 239(1):221-36.
6. Gat O., Grosfeld H., Ariel N. Search for Bacillus anthracis potential vaccine candidates by a functional genomic-serologic screen. Infect. Immun. 2006; 74(7):3987-4001.
7. Mignot T., Mock M., Fouet A. A plasmid-encoded regulator couples the synthesis of toxins and surface structures in Bacillus anthracis. Mol. Microbiol. 2003; 47(4):917-27.
8. Mock M., Fouet A. Anthrax. Microbiol. Rev. 2001; 55:647-71.
Review
For citations:
Popova P.Yu., Mikshis N.I., Goncharova A.Yu., Kashtanova T.N., Popov Yu.A. Peculiarities of Antibody Genesis in the Laboratory Animals Immunized with Anthrax Protective Antigen Preparation, Bacillus anthracis Recombinant or Bacillus anthracis Vaccine Strains. Problems of Particularly Dangerous Infections. 2012;(2(112)):75-77. (In Russ.) https://doi.org/10.21055/0370-1069-2012-2(112)-75-77
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