Production of Rabbit Anti-Rabies Immunoglobulin Using Cultural Antigen
https://doi.org/10.21055/0370-1069-2012-2(112)-78-81
Abstract
Justified is the possibility of application of fixed rabies virus Moscow 3253, reproduced on the cell culture Vero, as an antigen for heterologous anti-rabies immunoglobulin production. Application of the adjuvant - aluminium hydrate - is determined to be effective. Herein, by the day 73 post immunization, specific antibodies titer is ≥ 1:500 (wherein specific activity has been identified by means of neutralization test, carried out on white mice, and dot-blot immunoassay). The level of specific activity in experimental samples of anti-rabies immunoglobulin, isolated from rabbit immune serum, corresponds to 332 and 347 ME/ml. Physicochemical and biological properties of anti-rabies immunoglobulin, produced with the help of cultural antigen, fully comply with regulatory requirements specified for commercial preparation of heterologous anti-rabies immunoglobulin.
About the Authors
S. V. Generalov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
E. G. Abramova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
Zh. V. Matveeva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
I. M. Zhulidov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
O. A. Lobovikova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
L. V. Savitskaya
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
L. N. Minaeva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
M. V. Galkina
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
T. A. Mikheeva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
A. V. Komissarov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
M. N. Kireev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
A. K. Nikiforov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
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Review
For citations:
Generalov S.V., Abramova E.G., Matveeva Zh.V., Zhulidov I.M., Lobovikova O.A., Savitskaya L.V., Minaeva L.N., Galkina M.V., Mikheeva T.A., Komissarov A.V., Kireev M.N., Nikiforov A.K. Production of Rabbit Anti-Rabies Immunoglobulin Using Cultural Antigen. Problems of Particularly Dangerous Infections. 2012;(2(112)):78-81. (In Russ.) https://doi.org/10.21055/0370-1069-2012-2(112)-78-81
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