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Simple Liquid Nutrient Medium for Molecular Genetic Investigations of Francisella tularensis

https://doi.org/10.21055/0370-1069-2009-4(102)-66-67

Abstract

Proposed was the simple liquid nutrient medium for tularemia agent biomass growth with the aim to isolate DNA and antigens out of bacterial cells and to culture the tranformants of F.tularensis. Shown was that yeastrel contained complete set of amino acids and growth stimulating agents required for F.tularensis growth, except for cysteine.

Keywords


616.981.455:576.809.33

About the Authors

A. A. Lapin
State Research Center of Applied Microbiology and Biotechnology
Russian Federation


V. M. Pavlov
State Research Center of Applied Microbiology and Biotechnology
Russian Federation


A. N. Mokrievich
State Research Center of Applied Microbiology and Biotechnology
Russian Federation


L. V. Domotenko
State Research Center of Applied Microbiology and Biotechnology
Russian Federation


M. V. Khramov
State Research Center of Applied Microbiology and Biotechnology
Russian Federation


References

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For citations:


Lapin A.A., Pavlov V.M., Mokrievich A.N., Domotenko L.V., Khramov M.V. Simple Liquid Nutrient Medium for Molecular Genetic Investigations of Francisella tularensis. Problems of Particularly Dangerous Infections. 2009;(4(102)):66-67. (In Russ.) https://doi.org/10.21055/0370-1069-2009-4(102)-66-67

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ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)