Truncation of Lipopolysaccharide Core Decreases Yersinia pestis Virulence
https://doi.org/10.21055/0370-1069-2009-1(99)-50-51
Abstract
The isogenic set of Yersinia pestis strains including wild-type strain 231 and its mutants with deletions of the structural genes, wabD, waaL, waaQ, waaE, and hldE, responsible for core oligosaccharide synthesis was generated using site directed mutagenesis. It was shown that gradual decrease of the core-oligosaccharide length was accompanied by reduction of mutants' virulence in subcutaneously infected mice and guinea pigs.
About the Authors
S. V. Dentovskaya
State Research Center fo r Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
M. E. Platonov
State Research Center fo r Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
T. I. Kombarova
State Research Center fo r Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
G. M. Titareva
State Research Center fo r Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
I. V. Bakhteeva
State Research Center fo r Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
R. Z. Shaikhutdinova
State Research Center fo r Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
S. A. Ivanov
State Research Center fo r Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
A. P. Anisimov
State Research Center fo r Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
References
1. Ашмарин И.П., Воробьев А.А. Статистические методы в микробиологических исследованиях. Л.: Медгиз; 1962. С. 85-104.
2. Дентовская С.В., Платонов М.Е., Бахтеева И.В., Анисимов А.П. Наличие полной структуры кора липополисахарида необходимо для активации плазминогена возбудителя чумы. Пробл. особо опасных инф. 2007; 1(93):49-51.
3. Donnenberg M.S., Kaper J.B. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 1991; 59(12):4310-7.
4. Guinet F., Ave P., Jones L., Huerre M., Carniel E. Defective innate cell response and lymph node infiltration specify Yersinia pestis infection. PLoS ONE. 2008; 3(2):e1688
5. Knirel Y.A., Dentovskaya S.V., Bystrova O.V., Kocharova N.A., Senchenkova S.N., Shaikhutdinova R.Z. et al. Relationship of the lipopolysaccharide structure of Yersinia pestis to resistance to antimicrobial factors. Adv. Exp. Med. Biol. 2007; 603:88-96.
6. Kukkonen M., Suomalainen M., Kyllonen P., Lahteenmaki K., Lang H., Virkola R. et al. Lack of O-antigen is essential for plasminogen activation by Yersinia pestis and Salmonella enterica. Mol. Microbiol. 2004; 51(1):215-25.
7. Skurnik M., Peippo A., Ervela E. Characterization of the O-antigen gene clusters of Yersinia pseudotuberculosis and the cryptic O-antigen gene cluster of Yersinia pestis shows that the plague bacillus is most closely related to and has evolved from Y. pseudotuberculosis serotype O:1b. Mol. Microbiol. 2000; 37(2):316-30.
Review
For citations:
Dentovskaya S.V., Platonov M.E., Kombarova T.I., Titareva G.M., Bakhteeva I.V., Shaikhutdinova R.Z., Ivanov S.A., Anisimov A.P. Truncation of Lipopolysaccharide Core Decreases Yersinia pestis Virulence. Problems of Particularly Dangerous Infections. 2009;(1(99)):50-51. (In Russ.) https://doi.org/10.21055/0370-1069-2009-1(99)-50-51
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