Production of Monoclonal Antibodies against Outer Membrane Proteins of Burkholderia pseudomallei, Strain C-141
https://doi.org/10.21055/0370-1069-2008-4(98)-54-57
Abstract
Monoclonal antibodies (MAb) were produced against two B. pseudomallei high-purified membrane proteins with Mr 29 kDa (p29) and 45 kDa (p45). Monoclonal antibodies from culture supernatant fluids of 4F2 and 1G11 clones showed specific interaction with protein moiety of p29 both Burkholderia pseudomallei and Burkholderia mallei in ELISA and Western blotting. However, MAb of 3G4 clone were bound to the LPS-protein structures of these microbial cells. Analysis of interaction of Mabs from 4F2 and 1G11 clones with antigens of different lysates of pathogenic cells confirmed high specificity of these antibodies to p29 membrane protein of B. pseudomallei and B. mallei.
About the Authors
S. S. Vetchinin
State Research Center for Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
P. C. Kopylov
State Research Center for Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
N. V. Kiseleva
State Research Center for Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
A. M. Baranov
State Research Center for Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
E. V. Baranova
State Research Center for Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
E. V. Galkina
State Research Center for Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
A. I. Borzilov
State Research Center for Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
I. Y. Kalachev
Branch o f Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region
Russian Federation
Russian Federation
References
1. Авророва И.В., Пивень Н.Н., Жукова С.И. и др. Журн. микробиол. 2004; 5:29-33.
2. Викторов Д.В., Меринова Л.К., Алексеев В.В. и др. Мол. генет. 2005; 4:17-21.
3. Захарова И.Б., Викторов Д.В., Меринова О.А. и др. Пробл. особо опасных инф. 2006; 1(91):35-8.
4. Иванова О.А., Ганина Е.А., Борзилов А.И. и др. В кн.: Матер. VII межгос. науч.-практ. конф. государств-участников СНГ. Оболенск; 2006. С. 145-6.
5. Калачев И.Я., Борзилов А.И., Иванова О.А. и др. В кн. Матер. научн. конф. Предотвращение распростр. биол. оружия. The Cooper. Biol. Res. Ann. Rev. СПб; 2004. С. 42-4.
6. Пивень Н.Н., Рыбкин В.С., Плеханова Г.Н. и др. Журн. микробиол., эпидемиол. и иммунобиол. 2001; 1:29-33.
7. Anuntagool N., Rugdech P., Sirisinha S.J. Clin. Microbiol. 1993; 31:1232-6.
8. Fomsgaard A., Freudenberg M.A., Galanos C. J. Clin. Microbiol. 1990; 28:2627-31.
9. Gotoh N., White N.J., Chaowagul W. et al. Microbiology. 1994; 140:797-805.
10. Laemmli U.K. Nature. 1970; 227:680-5.
11. Nowinski R.C., Lostrom M.E., Tam M.R. Virology. 1979; 93:111-26.
12. Owen P., Freer J.H. Biochem. J. 1970; 120:237-43.
13. Pongsunk S., Thirawattanasuk N., Piyasangthong N. et al. J. Clin. Microbiol. 1999; 37:3662-7.
14. Westphal A., Lann H. Meth. Carbohydr. Chem. 1965; 5:83-91.
15. Wuthiekanun V.D., Dance A.B., Chaowagul W. et al. Eur. J. Clin. Microbiol. Infect. 1990; 9:654-8.
Review
For citations:
Vetchinin S.S., Kopylov P.C., Kiseleva N.V., Baranov A.M., Baranova E.V., Galkina E.V., Borzilov A.I., Kalachev I.Y. Production of Monoclonal Antibodies against Outer Membrane Proteins of Burkholderia pseudomallei, Strain C-141. Problems of Particularly Dangerous Infections. 2008;(4(98)):54-57. (In Russ.) https://doi.org/10.21055/0370-1069-2008-4(98)-54-57
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