Virulence for Mice of pH6+ and pH6– Yersinia pestis Strains
https://doi.org/10.21055/0370-1069-2008-1(95)-34-36
Abstract
The technique of site-directed mutagenesis of psa operon encoding the synthesis of pH6 antigen (adhesion pili), followed by the trans-complementation procedure were used to construct two sets of isogenic Yersinia pestis strains including wild type strains 231 and I-1996, their non-polar pH6- mutants containing deletions in the structural psA gene or in the entire operon, as well as the strains which could recover their capability for temperature- and pH-dependent synthesis of the adhesion pili or the constitutive pH6 antigen production. The loss of the ability to synthesize the pH6 antigen and its constitutive production was shown to affect neither their virulence characteristics nor mean lifetime assessments for the subcutaneously infected mice.
About the Authors
I. V. Bakhteyeva
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
S. V. Dentovskaya
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
E. A. Panfertsev
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
T. E. Svetoch
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
T. B. Kravchenko,
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
M. E. Platonov
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
G. M. Titareva
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
T. I. Kombarova
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
S. A. Ivanov
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
A. P. Anissimov
State Research Center of Applied Microbiology and Biotechnology, Obolensk
Russian Federation
Russian Federation
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Review
For citations:
Bakhteyeva I.V., Dentovskaya S.V., Panfertsev E.A., Svetoch T.E., Kravchenko, T.B., Platonov M.E., Titareva G.M., Kombarova T.I., Ivanov S.A., Anissimov A.P. Virulence for Mice of pH6+ and pH6– Yersinia pestis Strains. Problems of Particularly Dangerous Infections. 2008;(1(95)):34-36. (In Russ.) https://doi.org/10.21055/0370-1069-2008-1(95)-34-36
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