Effect of Bacteriophages and Gentamycine on Morphology and Vesicle Formation of Bacteria Yersinia pestis EV
https://doi.org/10.21055/0370-1069-2019-2-50-54
Abstract
Objective was to assess the effect of specific bacteriophages and gentamycine on the morphological-functional properties of bacteria in the vaccine strain Yersinia pestis EV.
Materials and methods. The vaccine strain Y. pestis EV, Pokrovskaya bacteriophage and the pseudotuberculous diagnostic bacteriophage were used for the study. The microbial culture was grown on solid and in liquid growth media at 27 °C for 20–24 h. The co-incubation of bacteria and bacteriophage or gentamycine was carried out at 27 °C for 20 minutes or at 37 °C for 2 hours, respectively. Culture preparations were examined by transmission electron microscopy.
Results and discussion. The influence of cultivation conditions and various stress factors on the vesicle production by the vaccine strain Y. pestis EV cells was evaluated. The nature and intensity of morphological-functional changes in Y. pestis EV cells in response to the effect of bacteriophages (plague Pokrovskaya and pseudotuberculous bacteriophages) or an antibiotic (gentamycine) were determined. It was established that co-incubation of Y. pestis EV with Pokrovskaya bacteriophage or gentamycine for 20 min leads to the increase in the production of extracellular vesicles and is accompanied by the development of degenerative changes in bacterial cells.
About the Authors
L. G. Dudina
Vyatka State University;
Komi Institute of Physiology at the Scientific Center of the Ural Branch of RAS
Russian Federation
Russian Federation
36, Moskovskaya St., Kirov, 610000;
Syktyvkar
M. A. Malkova
Vyatka State University;
Komi Institute of Physiology at the Scientific Center of the Ural Branch of RAS
Russian Federation
Russian Federation
36, Moskovskaya St., Kirov, 610000;
Syktyvkar
A. V. Chernyad’ev
Vyatka State University
Russian Federation
36, Moskovskaya St., Kirov, 610000
Russian Federation
36, Moskovskaya St., Kirov, 610000
S. G. Litvinets
Vyatka State University
Russian Federation
36, Moskovskaya St., Kirov, 610000
Russian Federation
36, Moskovskaya St., Kirov, 610000
A. A. Byvalov
Vyatka State University;
Komi Institute of Physiology at the Scientific Center of the Ural Branch of RAS
Russian Federation
Russian Federation
36, Moskovskaya St., Kirov, 610000;
Syktyvkar
References
1. World Health Organization Regional Office for Africa. Plague outbreak situation reports 2017. [Internet]. (Cited 6 Dec 2017). Available from: http://www.afro.who.int/health-topics/plague/plague-outbreak-situation-reports.
2. Cabanel N., Bouchier C., Rajerison M., Carniel E. Plasmidmediated doxycycline resistance in a Yersinia pestis strain isolated from a rat. Int. J. Antimicrob. Agents. 2018; 51(2):249–54. DOI: 10.1016/j.ijantimicag.2017.09.015.
3. Galimand M., Carniel E., Courvalin P. Resistance of Yersinia pestis to antimicrobial agents. Antimicrob. Agents Chemother. 2006; 50(10):3233–36. DOI: 10.1128/AAC.00306-06.
4. Welch T.J., Fricke W.F., McDermott P.F., White D.G., Rosso M.L., Rasko D.A., Mammel M.K., Eppinger M., Rosovitz M.J., Wagner D., Rahalison L., Leclerc J.E., Hinshaw J.M., Lindler L.E., Cebula T.A., Carniel E., Ravel J. Multiple antimicrobial resistance in plague: an emerging public health risk. PLoS ONE. 2007; 2(3):e309. DOI: 10.1371/journal.pone.0000309.
5. Filippov A.A., Sergueev K.V., He Y., Huang X.Z., Gnade B.T., Mueller A.J., Fernandez-Prada C.M., Nikolich M.P. Bacteriophage therapy of experimental bubonic plague in mice. Adv. Exp. Med. Biol. 2012; 954:337–48. DOI: 10.1007/978-1-4614-3561-7_41.
6. Manning A.J., Kuehn M.J. Contribution of bacterial outer membrane vesicles to innate bacterial defense. BMC Microbiol. 2011; 11:258. DOI: 10.1186/1471-2180-11-258.
7. Olsen I., Amano A. Outer membrane vesicles offensive weapons or good Samaritans? J. Oral Microbiol. 2015; 7:27468. DOI: 10.3402/jom.v7.27468.
8. Eddy J.L., Gielda L.M., Caulfield A.J., Rangel S.M., Lathem W.W. Production of Outer Membrane Vesicles by the Plague Pathogen Yersinia pestis. PLoS ONE. 2014; 9(9):e107002. DOI: 10.1371/journal.pone.0107002.
9. Kolodziejek A.M., Caplan A.B., Bohach G.A., Paszczynski A.J., Minnich S.A., Hovdea C.J. Physiological Levels of Glucose Induce Membrane Vesicle Secretion and Affect the Lipid and Рrotein Composition of Yersinia pestis Cell Surfaces. Appl Environ Microbiol. 2013; 79(14):4509–14. DOI: 10.1128/AEM.00675-13.
10. Reynolds E.S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 1963; 17:208– 12. PMID: 13986422. PMCID: PMC2106263.
11. Collins B.S. Gram-negative outer membrane vesicles in vaccine development. Discov. Med. 2011; 12(62):7–15. PMID: 21794204.
12. van Niel G., D’Angelo G., Raposo G. Shedding light on the cell biology of extracellular vesicles. Nat. Rev. Mol. Cell. Biol. 2018; 19(4):213–28. DOI: 10.1038/nrm.2017.125.
13. Klimentová J., Stulík J. Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria. Microbiol. Res. 2015; 170:1–9. DOI: 10.1016/j.micres.2014.09.006.
14. Jan A.T. Outer Membrane Vesicles (OMVs) of Gramnegative Bacteria: A Perspective Update. Front. Microbiol. 2017; 8:1053. PMID: 28649237. DOI: 10.3389/fmicb.2017.01053.
15. Anisimov A.P., Lindler L.E., Pier G B. Intraspecific Diversity of Yersinia pestis. Clin. Microbiol. Rev. 2004; 17(2):434– 64. DOI: 10.1128/CMR.17.2.434-464.2004.
16. Schwechheimer C., Kuehn M.J. Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions. Nat. Rev. Microbiol. 2015; 13(10):605–19. DOI: 10.1038/nrmicro3525.
17. Wang W., Chanda W., Zhong M. The relationship between biofilm and outer membrane vesicles: a novel therapy overview. FEMS Microbiol. Lett. 2015; 362(15):fnv117. DOI: 10.1093/femsle/fnv117.
18. Schachterle J.K., Stewart R.M., Schachterle M.B., Calder J.T., Kang H., Prince J.T., Erickson D.L. Yersinia pseudotuberculosis BarA-UvrY Two-Component Regulatory System Represses Biofilms via CsrB. Front. Cell. Infect. Microbiol. 2018; 8:323. DOI: 10.3389/fcimb.2018.00323. PMID: 30280093.
Review
For citations:
Dudina L.G., Malkova M.A., Chernyad’ev A.V., Litvinets S.G., Byvalov A.A. Effect of Bacteriophages and Gentamycine on Morphology and Vesicle Formation of Bacteria Yersinia pestis EV. Problems of Particularly Dangerous Infections. 2019;(2):50-54. (In Russ.) https://doi.org/10.21055/0370-1069-2019-2-50-54
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