The First Case of Yersinia Pestis Subsp. Pestis Isolation in the Territory of Altai Mountain Natural Plague Focus. Communication 1. Microbiological Characteristics, Molecular-Genetic and Mass-Spectrometric Identification of the Isolate
https://doi.org/10.21055/0370-1069-2013-1-60-65
Abstract
Yersinia pestis main ssp. strain, which was isolated for the first time in the history of surveillance over the Altai mountain natural plague focus in June, 2012. Determined is its high universal virulence. Plasmid screening, multi-locus VNTR- and mass-spectrometric analyses have revealed the strain to be more closely related to the plague agent variant, circulating in the territory of the natural focus Khuukh-Serkh-Munkh-Khairkhan, Bayan-Ul’giisk aimak, Mongolia.
Keywords
About the Authors
S. V. BalakhonovRussian Federation
M. V. Afanas’Ev
Russian Federation
M. Yu. Shestopalov
Russian Federation
A. S. Ostyak
Russian Federation
S. A. Vityazeva
Russian Federation
V. M. Korzun
Russian Federation
D. B. Verzhutsky
Russian Federation
E. P. Mikhailov
Russian Federation
A. I. Mishchenko
Russian Federation
A. V. Denisov
Russian Federation
N. I. Ivzhenko
Russian Federation
E. N. Rozhdestvensky
Russian Federation
E. N. Viskov
Russian Federation
L. A. Fomina
Russian Federation
References
1. Aparin G.P., Balakhonov S.V., Timofeeva L.A., Logachev A.I. [Numerical analysis of the phenotype properties and general genome characteristics of the plague agent strains pertaining to different subspecies]. Zh. Mikrobiol. Epidemiol. Immunobiol. 1987; 11:16–20.
2. Ashmarin I.P., Vorob’ev A.A. [Statistical Methods in Microbiological Investigations]. L.: MEDGIZ; 1962. 179 p.
3. Balabkin A.K., Shamova A.M., Sarzhinsky V.A., Lazarev B.V., Gorbacheva L.A. [About the natural plague focus in the territory of Gorny Altai]. [Reports of the Irkutsk Anti-Plague Institute]. 1962; 4:3–5.
4. Balakhonov S.V., Verxhutsky D.B., Korzun V.M., Vershinin E.A., Nemchenko L.S., Chipanin E.V. [Current state of the natural plague foci in Siberia]. Zh. Infek. Patol. 2009; 16(3):16–20.
5. Balakhonov S.V., Tsendzhav S., Erdenebat A. [New plasmidovars of the plague agent strains, isolated in Mongolia]. Molek. Genet. 1991; 11:27–9.
6. Balakhonov S.V., Shestopalov M.Yu., Romanova I.F. [Results of the VNTR-analysis for (5’CAAA-3’)n locus of Yersinia pestis strains, isolated from active natural plague foci of Siberia]. Molek. Genet. 2009; 3:14–7.
7. Logachev A.I., Korzun V.M., Mikhailov E.P., Rozhdestvensky E.N., Balakhonov S.V. [Distribution of tryptophan-dependent Yersinia pestis variants in the Altai mountain natural plague focus]. Probl. Osobo Opasn. Infek. 2012; (113):20–5.
8. Onishchenko G.G., Kutyrev V.V., editors. [Laboratory Diagnostics of Particularly Dangerous Infections: Practice Guidelines]. M.: Meditsina, Shiko; 2009. P. 27–60.
9. Tarasova V.E. [Concerning detection of plague agent in rodents in the territory of Gorny Altai]. [Reports of the Irkutsk Anti-Plague Institute]. 1962; 3:11–3.
10. Erdenebat A., Batbold J., Dashnyam B. Plasmid content and distribution of Y. pestis isolated in Mongolia. Scientific Journal. Center for Infectious Diseases With Natural Foci. 2001; 9:187–91.
11. Le Flèche P., Hauck Y., Onteniente L., Prieur A., Denoeud F., Ramisse V., Sylvestre P., Benson G., Ramisse F., Vergnaud G. A tandem repeats database for bacterial genomes: application to the genotyping of Yersinia pestis and Bacillus anthracis. BMC Microbiol. 2001; 1:2.
12. Marklein G., Josten M., Klanke U., Muller E., Horre R., Maier T., Wensel T., Kostrzewa M., Bierbaum G., Hoerauf A. Matrix assisted laser desorption ionization-time of flight mass-spectrometry for fast and reliable identification of clinical yeast isolates. J. Clin. Microbiol. 2009; 47:2912–17.
Review
For citations:
Balakhonov S.V., Afanas’Ev M.V., Shestopalov M.Yu., Ostyak A.S., Vityazeva S.A., Korzun V.M., Verzhutsky D.B., Mikhailov E.P., Mishchenko A.I., Denisov A.V., Ivzhenko N.I., Rozhdestvensky E.N., Viskov E.N., Fomina L.A. The First Case of Yersinia Pestis Subsp. Pestis Isolation in the Territory of Altai Mountain Natural Plague Focus. Communication 1. Microbiological Characteristics, Molecular-Genetic and Mass-Spectrometric Identification of the Isolate. Problems of Particularly Dangerous Infections. 2013;(1):60-65. (In Russ.) https://doi.org/10.21055/0370-1069-2013-1-60-65