Modern Methods of DNA Sequencing (Scientific Review)
https://doi.org/10.21055/0370-1069-2014-2-73-79
Abstract
Rapidly developing sequencing technologies have provided the possibility for identification of a DNA nucleotide sequence of a whole individual human genome just in a couple of weeks. Working capacity of some sequencers is already measured in thousands of milliards of base pairs per an operating cycle. Reviewed have been the basic principles and analytical potential of the modern methods for DNA sequencing which are nominally subdivided into three major types: classical - capillary electrophoresis sequencing and pyrosequencing, novel (Next Generation Sequencing - NGS) - simultaneous sequencing of millions of DNA fragments, each one of which is a cluster containing many thousands or hundreds of thousands of their clones - high-performance pyrosequencing, cyclic ligase and semiconducting sequencing, molecular-cluster-based sequencing using fluorescent-labeled precursors; and cutting-edge methods - (Next-Next Generation Sequencing - NNGS) - the ones that read millions of single DNA fragments without pre-cloning.
About the Authors
Ya. M. Krasnov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. P. Guseva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. A. Sharapova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Cherkasov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Krasnov Ya.M., Guseva N.P., Sharapova N.A., Cherkasov A.V. Modern Methods of DNA Sequencing (Scientific Review). Problems of Particularly Dangerous Infections. 2014;(2):73-79. (In Russ.) https://doi.org/10.21055/0370-1069-2014-2-73-79
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