Application of the Rapid Linear Immune-Electrophoresis for Differentiation between Burkholderia
https://doi.org/10.21055/0370-1069-2014-2-94-96
Abstract
Objective of the study was to develop a method that would allow for rapid linear immune-electrophoresis to differentiate between pathogenic agents of glanders and melioidosis and non-pathogenic closely-related Burkholderia . The put-forward modification of the technique made it possible to detect the antigens of pathogenic B. pseudomallei and B. mallei due to the presence of precipitation lines in between the sample gel and the one with immune sera. B. thailandensis, B. cepacia, and B. gladioli did not form such precipitation lines, which in its turn provided for the possibility to differentiate between the mentioned ones and pathogenic Burkholderia. The rapid enhanced linear immune-electrophoresis is easy to perform and compelling, and takes little time. It is qualified for identification of heterogenic and specific antigens in Burkholderia , selection of immune sera containing antibodies to the existing antigens, and can be used as a supportive alternative analytical means for the detection of antigens of pathogenic Burkholderia .
About the Authors
I. I. Korsakova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
N. P. Khrapova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
G. M. Napalkova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
L. V. Lomova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
T. V. Bulatova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
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Review
For citations:
Korsakova I.I., Khrapova N.P., Napalkova G.M., Lomova L.V., Bulatova T.V. Application of the Rapid Linear Immune-Electrophoresis for Differentiation between Burkholderia. Problems of Particularly Dangerous Infections. 2014;(2):94-96. (In Russ.) https://doi.org/10.21055/0370-1069-2014-2-94-96
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