Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture
https://doi.org/10.21055/0370-1069-2014-2-101-103
Abstract
/cell, using maintenance media 199 with admixture of 0.1 % human serum albumin or 2 % bovine serum. Optimum media volume in the roll-bottle for fixed rabies virus strain, “Moscow 3253”, cultivation is 200-400 ml. It depends upon the proliferating surface area. Specifications stated above provide for the obtainment of culture liquid with rabies virus titer - 1:256 - 1:512, if assayed in ELISA. This cultural virus is recommended as a basis for immunization material obtainment with a view to produce anti-rabies immunoglobulin from equine blood serum.
About the Authors
S. V. Generalov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
E. G. Abramova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
Zh. V. Matveeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
I. M. Zhulidov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. V. Savitskaya
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. A. Lobovikova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Generalov S.V., Abramova E.G., Matveeva Zh.V., Zhulidov I.M., Savitskaya L.V., Lobovikova O.A. Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture. Problems of Particularly Dangerous Infections. 2014;(2):101-103. (In Russ.) https://doi.org/10.21055/0370-1069-2014-2-101-103
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