Characteristics and Philogenetic Analysis of Yersinia pseudotuberculosis Strains from the Sarydzhaz High-Mountain Focus in the Tien-Shan
A. K. Dzhaparova, 
G. A. Eroshenko,
K. A. Nikiforov,
L. M. Kukleva,
Zh. V. Al’khova,
S. K. Berdiev,
V. V. Kutyrev
G. A. Eroshenko,
K. A. Nikiforov,
L. M. Kukleva,
Zh. V. Al’khova,
S. K. Berdiev,
V. V. Kutyrev https://doi.org/10.21055/0370-1069-2021-2-87-93
Abstract
The aim of the study was to characterize and carry out molecular-genetic identification of Yersinia pseudotuberculosis strains isolated in the Sarydzhaz high-mountain focus of the Tien-Shan.
Materials and methods. The Y. pestis and Y. pseudotuberculosis cultures were differentiated according to their sensitivity to diagnostic bacteriophages, motility, urea fermentation, and pesticin production. Whole genome sequencing was performed using the Ion S5 XL System (Thermo Fischer Scientific). Ion Torrent Suite software package, 5.12 and Newbler gsAssembler 2.6 were used for data processing and de novo assembly of raw read sequences. The dendrogram was constructed using the Maximum Likelihood method applying the PhyML 3.1 software.
Results and discussion. Strains of Y. pseudotuberculosis were isolated from museum mixed with Y. pestis cultures obtained from marmots in the Sarydzhaz high-mountain focus. According to whole genome SNP analysis, they belong to the phylogenetic group O:3 serovar, which is widespread throughout the world. Y. pseudotuberculosis is often present in mixed with Y. pestis cultures, isolated from rodents, which should be taken into account when carrying out laboratory diagnostics of plague in natural foci.
About the Authors
A. K. Dzhaparova
Republican Center of Quarantine and Particularly Dangerous Infections
Russian Federation
Russian Federation
92, Skryabina St., Bishkek
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
K. A. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
Zh. V. Al’khova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
S. K. Berdiev
Republican Center of Quarantine and Particularly Dangerous Infections
Russian Federation
Russian Federation
92, Skryabina St., Bishkek
V. V. Kutyrev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
References
1. Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T., Prentice M.B., Sebaihia M., James K.D., Churcher C., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., CerdeñoTárraga A.M., Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C., Quail M., Rutherford K., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Genome sequence of Yersinia pestis, the causative agent of plague. Nature. 2001; 413(6855):523–7. DOI: 10.1038/35097083.
2. Skurnik M., Peippo A., Ervelä E. Characterization of the O-antigen gene clusters of Yersinia pseudotuberculosis and the cryp- tic O-antigen gene cluster of Yersinia pestis shows that the plague bacillus is most closely related to and has evolved from Y. pseudotu- berculosis serotype O:1b. Mol. Microbiol. 2000; 37(2):316–30. DOI: 10.1046/j.1365-2958.2000.01993.x.
3. Wren B.W. The Yersiniae – a model genus to study the rapid evolution of bacterial pathogens. Nat. Rev. Microbiol. 2003; 1(1):55– 64. DOI: 10.1038/nrmicro730.
4. Chain P.S., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B., Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis. Proc. Natl Acad. Sci. USA. 2004; 101(38):13826–31. DOI: 10.1073/pnas.0404012101.
5. Reuter S., Connor T.R., Barquist L., Walker D., Feltwell T., Harris S.R., Fookes M., Hall M.E., Petty N.K., Fuchs T.M., Corander J., Dufour M., Ringwood T., Savin C., Bouchier C., Martin L., Miettinen M., Shubin M., Riehm J.M., Laukkanen-Ninios R., Sihvonen L.M., Siitonen A., Skurnik M., Falcão J.P., Fukushima H., Scholz H.C., Prentice M.B., Wren B.W., Parkhill J., Carniel E., Achtman M., McNally A., Thomson N.R. Parallel independent evo- lution of pathogenicity within the genus Yersinia. Proc. Natl Acad. Sci. USA. 2014; 111(18):6768–73. DOI: 10.1073/pnas.1317161111.
6. McNally A., Thomson N.R., Reuter S., Wren B.W. Add, stir and reduce: Yersinia spp. as model bacteria for pathogen evo- lution. Nat. Rev. Microbiol. 2016; 14(3):177–90. DOI: 10.1038/nrmicro.2015.29.
7. Tsubokura M., Aleksić S. A simplified antigenic scheme for serotyping of Yersinia pseudotuberculosis: phenotypic character- ization of reference strains and preparation of O and H factor sera. Contrib. Microbiol. Immunol. 1995; (13):99–105.
8. Gemski O.P., Lazere J.R., Casey T., Wohlhieter J.A. Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis. Infect. Immun. 1980; 28(3):1044–7. DOI: 10.1128/iai.28.3.1044-1047.1980.
9. Gemski P., Lazere J.R., Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect. Immun. 1980; 27(2):682–5. DOI: 10.1128/iai.27.2.682-685.1980.
10. Portnoy D.A., Martinez R.J. Role of a plasmid in the patho- genicity of Yersinia species. Curr. Top. Microbiol. Immunol. 1985; 118:29–51. DOI: 10.1007/978-3-642-70586-1_3.
11. Kutyrev V.V., Popov Yu.A., Protsenko O.A. [Plasmids of pathogenicity of Yersinia pestis]. Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya [Molecular Genetics, Microbiology and Virology]. 1986; 6:3–11.
12. Eroshenko G.A., Nosov N.Y., Krasnov Y.M., Oglodin Y.G., Kukleva L.M., Guseva N.P., Kuznetsov A.A., Abdikarimov S.T., Dzhaparova A.K., Kutyrev V.V. Yersinia pestis strains of ancient phylogenetic branch 0.ANT are widely spread in the high-mountain plague foci of Kyrgyzstan. PLoS One. 2017; 12(10):e0187230. DOI: 10.1371/journal.pone.0187230.
13. Kutyrev V.V., Eroshenko G.A., Motin V.L., Nosov N.Y., Krasnov J.M., Kukleva L.M., Nikiforov K.A., Al’hova Zh.V., Oglodin E.G., Guseva N.P. Phylogeny and classification of Yersinia pestis through the lens of strains from the plague foci of Commonwealth of Independent States. Front. Microbiol. 2018; 9:1106. DOI: 10.3389/fmicb.2018.01106.
14. Eroshenko G.A., Dzhaparova A.K., Oglodin E.G., Al’khova Zh.V., Kukleva L.M., Kuznetsov A.A., Krasnov Ya.M., Abdikarimov S.T., Kutyrev V.V. [Phylogeny of Yersinia pestis Strains Belonging to 0.ANT Branch, Isolated in Tien-Shan and Pamir-Alay in XX– XXI Centuries]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2020; (1):76–84. DOI: 10.21055/0370-1069-2020-1-76-84.
15. Onishchenko G.G., Kutyrev V.V., editors. [Laboratory Diagnostics of Particularly Dangerous Infectious Diseases. Practice Guidelines]. Moscow: “Shiko”; 2013. 560 p.
16. Platonov M.E., Blouin Y., Evseeva V.V., Afanas’ev M.V., Pourcel C., Balakhonov S.V., Vergnaud G., Anisimov A.P. Draft ge- nome sequences of five Yersinia pseudotuberculosis ST19 isolates and one isolate variant. Genome Announc. 2013; 1(2):e0012213. DOI: 10.1128/genomeA.00122-13.
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For citations:
Dzhaparova A.K., Eroshenko G.A., Nikiforov K.A., Kukleva L.M., Al’khova Zh.V., Berdiev S.K., Kutyrev V.V. Characteristics and Philogenetic Analysis of Yersinia pseudotuberculosis Strains from the Sarydzhaz High-Mountain Focus in the Tien-Shan. Problems of Particularly Dangerous Infections. 2021;(2):87-93. (In Russ.) https://doi.org/10.21055/0370-1069-2021-2-87-93
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