Results of Modeling Experiments in Designing Immuno-Enzyme Test-System for the Detection of Antibodies to Yersinia pestis F1 (ELISA-Ab-F1 Yersinia pestis)
https://doi.org/10.21055/0370-1069-2013-1-74-77
Abstract
Designed is immuno-enzyme test-system for the detection of antibodies to Yersinia pestis capsular antigen F1 – “ELISA-Ab-F1
Yersinia pestis”. On the model of laboratory mice it is demonstrated that this test-system is highly specific, its diagnostic titer being 1/320.Diagnostic value of the test-system is 83.3–88.9 % as revealed through investigations of sera and blood suspension samples, swabs of thoracic organs of animals, inoculated with live plague vaccine, strains of plague microbe, containing and deprived of pFra, as well as with heterologous bacteria.
Yersinia pestis”. On the model of laboratory mice it is demonstrated that this test-system is highly specific, its diagnostic titer being 1/320.Diagnostic value of the test-system is 83.3–88.9 % as revealed through investigations of sera and blood suspension samples, swabs of thoracic organs of animals, inoculated with live plague vaccine, strains of plague microbe, containing and deprived of pFra, as well as with heterologous bacteria.
About the Authors
Z. L. Devdariani
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. E. Tereshkina
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
T. M. Taranenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
M. N. Kireev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
I. V. Terekhova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
G. V. Grigor’Eva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
M. N. Islyaeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. M. Ermakov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. A. Vinogradova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. N. Malakhaeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Devdariani Z.L., Tereshkina N.E., Taranenko T.M., Kireev M.N., Terekhova I.V., Grigor’Eva G.V., Islyaeva M.N., Ermakov N.M., Vinogradova N.A., Malakhaeva A.N. Results of Modeling Experiments in Designing Immuno-Enzyme Test-System for the Detection of Antibodies to Yersinia pestis F1 (ELISA-Ab-F1 Yersinia pestis). Problems of Particularly Dangerous Infections. 2013;(1):74-77. (In Russ.) https://doi.org/10.21055/0370-1069-2013-1-74-77
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