Optimization of the Conditions for Cultivation of Yersinia pseudotuberculosis in the Process of Obtaining Cell Mass
https://doi.org/10.21055/0370-1069-2021-4-137-142
Abstract
Objective of the study was to optimize the conditions of submerged recurrent cultivation and the composition of the nutrient medium for obtaining the cell mass of Yersinia pseudotuberculosis strains, used as an adsorbent in the preparation of diagnostic plague immunoglobulins. Materials and methods. We utilized Y. pseudotuberculosis adsorbent strains 6; 31; 68; 69; and 70 belonging to V, I, III, IV, and V serotypes, respectively, received from the State Collection of Pathogenic Bacteria at the premises of the RusRAPI “Microbe”. The cultivation process was carried out on an incubator-shaker, laboratory and pilot fermenters with variation of the process parameters, different options for feeding and nutrient media. Results and discussion. In the course of work, the optimal parameters of recurring submerged cultivation have been established. It was found that the highest biomass yield is provided by a combination of a nutrient medium – a carbon substrate in the form of a broth, based on an enzymatic fibrin hydrolysate, with the addition of galactose as a substrate feeding. Thereat, the morphology and immunochemical properties of microbial cells obtained through modified preparation process do not differ from those produced in the control run. The optimization of the parameters for Y. pseudotuberculosis cell mass cultivation with subsequent upscaling has been performed.
About the Authors
R. R. Salikhov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
S. V. Borisova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
N. G. Avdeeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Yu. I. Samokhvalova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
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Review
For citations:
Salikhov R.R., Borisova S.V., Avdeeva N.G., Samokhvalova Yu.I., Volokh O.A. Optimization of the Conditions for Cultivation of Yersinia pseudotuberculosis in the Process of Obtaining Cell Mass. Problems of Particularly Dangerous Infections. 2021;(4):137-142. (In Russ.) https://doi.org/10.21055/0370-1069-2021-4-137-142
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