Development of a Set of Primers for Drug-Resistance Genes Detection in the Agents of Dangerous Bacterial Infections as Exemplified by Yersinia pestis, Vibrio cholerae, Escherichia coli Strains
https://doi.org/10.21055/0370-1069-2014-3-57-60
Abstract
A set of primers for detection of genes encoding resistance to streptomycin ( strA, strB ), tetracyclin ( tetA, tetR ), chloramphenicol ( catА ), kanamycin ( npt , aphA ), vankomycin ( sanA ), polymyxin ( pmrD ) has been developed with the aim of rapid and effective detection of drug-resistant strains of dangerous bacterial infections agents. Efficacy of constructed primers has been confirmed against a panel of 40 Yersinia pestis, 49 Vibrio cholerae, and 2 Escherichia coli strains from the State collection of pathogenic bacteria of the RAPI “Microbe”. Drug-resistance genes ntp and catA have been detected in plague agent strains , strA, strB , npt , aphA , tetA and tetR - in cholera agent; strA , tetR , ntp and aphA - in pathogenic strain E. coli О157:H7. Determined is universal character of the designed primers for drug-resistance genes detection in these pathogenic bacteria species.
About the Authors
K. A. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. V. Anisimova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
G. N. Odinokov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Fadeeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. A. Novichkova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
V. V. Kutyrev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Nikiforov K.A., Anisimova L.V., Odinokov G.N., Fadeeva A.V., Novichkova L.A., Eroshenko G.A., Kutyrev V.V. Development of a Set of Primers for Drug-Resistance Genes Detection in the Agents of Dangerous Bacterial Infections as Exemplified by Yersinia pestis, Vibrio cholerae, Escherichia coli Strains. Problems of Particularly Dangerous Infections. 2014;(3):57-60. (In Russ.) https://doi.org/10.21055/0370-1069-2014-3-57-60
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