Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results
https://doi.org/10.21055/0370-1069-2014-4-52-55
Abstract
For the first time ever developed has been the method of identification of genovariants in Yersinia pestis strains belonging to the main subspecies using PCR with hybridization-fluorescent registration of results. A complex of DNA-targets, the most of which are newly detected ones, have been utilized to differentiate between genovariants of antique (0.ANT, 1.ANT, 2.ANT, 4.ANT), medievalis (2.MED, 2.MED0), and orientalis (1.ORI) biovars. Application of these targets in PCR with hybridization-fluorescent registration of results provides for a rapid, effective and reliable classifying of the strains of the genovariants, circulating in various geographical regions. Efficacy of the method has been validated via analysis of 110 Y. pestis strains, isolated in the territory of the Russian Federation, neighboring countries and beyond, including 37 strains of antique, 53 strains of medievalis, and 20 strains of orientalis biovars.
About the Authors
E. G. Oglodin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
G. N. Odinokov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
K. A. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
References
1. Eroshenko G.A., Odinokov G.N., Kukleva L.M., Shavina N.Yu., Guseva N.P., Kutyrev V.V. [Genetic foundations of the variability of nitrate reduction in Yersinia pestis strains]. Genetika. 2014; 50:1–9.
2. Zharinova N.V., Bryukhanova G.D., Eremenko E.I., Bryukhanov A.F., Beyer A.P., Tsareva N.S., Zaitsev A.A. [Properties of the plague agent strains circulating in Central-Caucasian high-mountain natural focus of plague]. Aktual. Probl. Epidemiol. Profilakt. Infek. Bol. 2004; 2:14.
3. Odinokov G.N., Eroshenko G.A., Krasnov Ya.M., Kukleva L.M., Cherkasov A.V., Shavina N.Yu., Kutyrev V.V. [Analysis of the genome wide sequence of Yersinia pestis strains based on the consecutive 680-SNP algorithm]. Probl. Osobo Opasn. Infek. 2013; 3:49–54.
4. [Management of the laboratories using methods of nucleic acids amplification when working with the materials containing microorganisms of the I–IV groups of pathogenicity. Methodological Regulations]. MR 1.3.2569-09.
5. Protsenko S.L., Serdyukova T.V., Rozanova G.N. [Detection of new plasmid in proline-dependent plague agent strains found in Central-Caucasian natural focus and heterogeneity in plasmid composition of the circulating in the focus strains]. In: [Particularly dangerous infections in the Caucasis]. Stavropol, 1987; 265–7.
6. Achtman M., Zurth K., Morelli G., Torrea G., Guiyoule A., Carniel E. Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis. Proc Natl Acad Sci USA. 1999; 96(24):14043.
7. Cui Y., Yu C., Yan Y., Li D., Li Y., Jombart T., Weinert L.A., Wang Z., Guo Z., Xu L., Zhang Y., Zheng H., Qin N., Xiao X., Wu M., Wang X., Zhou D., Qi Z., Du Z., Wu H., Yang X., Cao H., Wang H., Wang J., Yao S., Rakin A., Li Y., Falush D., Balloux F., Achtman M., Song Y., Wang J., Yang R. Historical variations in mutational rate in an epidemic pathogen Yersinia pestis. Proc Natl Acad Sci USA. 2013; 110(2):577.
8. Morelli G., Song Y., Mazzoni C.J., Eppinger M., Raumagnac P., Wagner D.V., Feldcamp M., Kusecek B., Vogler A.J., Li Y., Cui Y., Thomson N.R., Jombart T., Leblois R. Phylogenetic diversity and historical patterns of pandemic spread of Yersinia pestis. Nat. Genet. 2010; 42(12):1140.
9. Motin V.L., Georgescu A.M., Elliott J.M., Hu P., Worsham P.L., Ott L.L., Slezak T.R., Sokhansanj B.A., Regala W.M., Brubaker R.R., Garcia E. Genetic variability of Yersinia pestis isolates as predicted by PCR-based IS100 genotyping and analysis of structural genes encoding glycerol-3-phosphate dehydrogenase (glpD). J. Bacteriol. 2002; 184(4):1019–27.
Review
For citations:
Oglodin E.G., Odinokov G.N., Nikiforov K.A., Kukleva L.M., Eroshenko G.A. Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results. Problems of Particularly Dangerous Infections. 2014;(4):52-55. (In Russ.) https://doi.org/10.21055/0370-1069-2014-4-52-55
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