Hardware-Controlled Method of Desalting Antigen Components of Cholera Chemical Vaccine
https://doi.org/10.21055/0370-1069-2014-4-61-64
Abstract
Experimentally substantiated is the possibility to apply tangential ultrafiltration for desalting antigen components of the tableted bivalent chemical cholera vaccine. Specified are the technological parameters of the process. It is demonstrated that the properties of choleragen-anatoxin (produced by Vibrio cholerae strain 569B Inaba) and O-antigens (produced from V. cholerae 569B Inaba and M-41 Ogawa strains) obtained using the designed methodology are as efficient as the ones manufactured using certified procedure and satisfy regulatory requirements. Experimentally substantiated technology for the desalting of antigen components of chemical cholera vaccine provides for the reduction of the time elapsed up to 5-6 hours from the original 3 to 4 days. It also allows for the manufacturing under controlled conditions. This hard-ware controlled method of desalting has been implemented into the vaccine production practice.
About the Authors
A. V. Komissarov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. K. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. I. Perepelitsa
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Eremin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. V. Gromova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
Yu. G. Vasin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Gaeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. F. Livanova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
References
1. Brakht K., Katalevsky E.E., Savel’eva S.P. [Cross-flow filtration]. Farmatsevt. Tekhnol. Upakovka. 2009; 6:47–51.
2. Komissarov A.V., Eremin S.A., Ul`yanov A.Yu., Aleshina Yu.A., Nikiforov A.K., Vasin Yu.G., Klokova O.D., Belyakova N.I. [Development of the experimental technology for protective antigens of Vibrio cholerae 569B Inaba concentration by means of tangential ultrafiltration]. Probl. Osobo Opasn. Infek. 2011; 3(109):75–7.
3. Komissarov A.V., Eremin S.A., Aleshina Yu.A., Vasin Yu.G., Klokova O.D., Beliakova N.I. [Experimental evaluation of application of “cross-flow” ultrafiltration method for O-antigen concentrating in cholera chemical bivalent vaccine production]. Probl. Osobo Opasn. Infek. 2011; 2(108):83–6.
4. Kutyrev V.V., Devdariani Z.L., Sayapina L.V. [Present status of the researches in the sphere of vaccine prophylaxis of particularly dangerous bacterial infections]. Probl. Osobo Opasn. Infek. 2006; 2(92):18–24.
5. [Methods of control of medical immunobiological preparations administered to humans. Methodological Regulations]. MR 4.1/4.2.588-96. M., 1998.
6. Onishchenko G.G., Kutyrev V.V., Shchukovskaya T.N., Smirnova N.I., Nikiforov A.K., Eremin S.A., Toporkov V.P. [Cholera specific prophylaxis in modern conditions]. Probl. Osobo Opasn. Infek. 2011; 1(107):5–12.
7. Cherkasov A.N., Pasechnik V.A. [Membranes and Sorbents in Biotechnology]. L.: Khimiya; 1991. 240 p.
8. Shchukovskaya T.N., Sayapina L.V., Kutyrev V.V. [Preventive vaccination against cholera: Current state of affairs]. Epidemiol. Vaktsinoprof. 2009; 2:62–7.
Review
For citations:
Komissarov A.V., Nikiforov A.K., Perepelitsa A.I., Eremin S.A., Gromova O.V., Vasin Yu.G., Volokh O.A., Gaeva A.V., Livanova L.F. Hardware-Controlled Method of Desalting Antigen Components of Cholera Chemical Vaccine. Problems of Particularly Dangerous Infections. 2014;(4):61-64. (In Russ.) https://doi.org/10.21055/0370-1069-2014-4-61-64
Views:
578
Email this article 