Intraspecific Differentiation of Yersinia pestis Using Mass Spectrometric Analysis
A. S. Abdrashitova,
A. V. Boiko,
N. E. Shcherbakova,
E. A. Bil’ko,
O. A. Koreshkova,
N. S. Chervyakova https://doi.org/10.21055/0370-1069-2025-3-86-92
Abstract
The aim of the study was to evaluate approaches and possibilities for differentiating strains of plague agent of the main and non–main subspecies using the method of mass spectrometric analysis.
Materials and methods. 102 strains of Yersinia pestis of 4 subspecies were used in the current work, which were grown on LB agar pH (7.2±0.1) at a temperature of (28±1) °C for (48±1) hours. The mass spectra of the samples were taken automatically with a laser frequency of 60 Hz on a Microflex LT mass spectrometer (Bruker Daltonics, Germany). The spectra were analyzed in the mass range of 2–20 kDa.
Results and discussion. Various approaches to the differentiation of plague microbe strains by subspecies (biovars) using the MALDI-TOF mass spectrometry method have been considered. When using visual analysis for the sample under study, it is not possible to select fragments of the proteinogram that could be considered specific signals for each subspecies or biovar of Y. pestis. Using the cluster analysis of the MALDI Biotyper program, the formation of two separate clusters was noted, including the mass spectra of strains of the main and Caucasian subspecies of the plague microbe. The mass spectra of Y. pestis strains of the central asiatica and ulegeica subspecies are not grouped into separate clusters. When evaluating the informative value of peaks in the mass spectra, it was demonstrated that the same peak has a different weight value for different subspecies (biovars). Thus, the possibility of using different approaches in the analysis of protein profiles of Y. pestis strains for their differentiation by subspecies and/or biovars has been examined.
About the Authors
A. S. Abdrashitova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
A. V. Boiko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
N. E. Shcherbakova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
E. A. Bil’ko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
O. A. Koreshkova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
N. S. Chervyakova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005
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Review
For citations:
Abdrashitova A.S., Boiko A.V., Shcherbakova N.E., Bil’ko E.A., Koreshkova O.A., Chervyakova N.S. Intraspecific Differentiation of Yersinia pestis Using Mass Spectrometric Analysis. Problems of Particularly Dangerous Infections. 2025;(3):86-92. (In Russ.) https://doi.org/10.21055/0370-1069-2025-3-86-92
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