Genetic Heterogeneity of Francisella tularensis Subspecies mediasiatica Strains
V. M. Sorokin,
N. V. Pavlovich,
M. V. Tsimbalistova,
N. V. Aronova,
A. S. Vodop’yanov,
R. V. Pisanov https://doi.org/10.21055/0370-1069-2025-3-154-159
Abstract
The aim of the study was to conduct comparative phylogenetic analysis of Francisella tularensis subsp. mediasiatica strains isolated in different territories of contemporary Republics of Kazakhstan, Uzbekistan, Altai Territory, and the Altai Republic in the period between 1960 and 2024.
Materials and methods. We used 77 F. tularensis strains isolated in the territories of Kazakhstan, Uzbekistan, Altai Territory and the Altai Republic of the Russian Federation in the period from 1960 to 2024. To determine the subspecies, the INDEL typing method was used. VNTR genotyping was carried out at 5 loci. Identification of clonal complexes was carried out using the MST method.
Results and discussion. Comparative analysis of VNTR genotypes of 42 F. tularensis subsp. mediasiatica strains circulating in natural foci of Altai between 2011 and 2024 made it possible to identify three clonal complexes (CC1-CC3), represented by 13, 14 and 7 individual genotypes, respectively. Strains of the “Kazakhstan” population of F. tularensis subsp. mediasiatica are represented by a separate clonal complex CC4. It has been established for the first time that the “Altai” population of F. tularensis strains of the subspecies mediasiatica is represented by three clonal complexes CC1-CC3. In the same territories of the Altai region, strains of different clonal complexes can circulate at different times. High genetic diversity of the Altai population subsp. mediasiatica was noted (Nei diversity index DI = 0.991). For the first time, a comparable phylogenetic analysis of the “Kazakhstan” and “Altai” populations of the causative agent of tularemia subsp. mediasiatica has been carried out. It is shown that the “Kazakhstan” population forms a separate clonal complex CC4 and it is connected with the Altai clonal complexes through different clones. The results of genotyping indicate the possible origin of the “Altai” population of mediasiatica subspecies.
About the Authors
V. M. Sorokin
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
117/40, M. Gor’kogo St., Rostov-on-Don, 344002
N. V. Pavlovich
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
117/40, M. Gor’kogo St., Rostov-on-Don, 344002
M. V. Tsimbalistova
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
117/40, M. Gor’kogo St., Rostov-on-Don, 344002
N. V. Aronova
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
117/40, M. Gor’kogo St., Rostov-on-Don, 344002
A. S. Vodop’yanov
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
117/40, M. Gor’kogo St., Rostov-on-Don, 344002
R. V. Pisanov
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
117/40, M. Gor’kogo St., Rostov-on-Don, 344002
References
1. Keim P., Johansson A., Wagner D.M. Molecular epidemiology, evolution, and ecology of Francisella. Ann. N. Y. Acad. Sci. 2007; 1105:30–66. DOI: 10.1196/annals.1409.0111.
2. Aikimbaev M.A. Taxonomy of the genus Francisella. Rep. Acad. Sci. Kaz. SSR Ser. Biol. 1966; 5:42–4.
3. Olsufjev N.G., Meshcheryakova I.S. Subspecific taxonomy of Francisella tularensis McCoy and Chapin 1912. Int. J. Syst. Bacteriol. 1983; 33(4):872–4. DOI: 10.1099/00207713-33-4-87221.
4. Timofeev V., Bakhteeva I., Mokrievich A., Vakhrameeva G., Gritskova E., Anisimov Yu., Rozhdestvensky E., Bazarova G., Zhumakaev R., Dyatlov I., Vergnaud G. The first finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an update of the subspecies genetic diversity. Bacteria. 2022; 1(4):242–49. DOI: 10.3390/bacteria1040018.
5. Mokrievich A.N., Timofeev V.S., Kudryavceva T.Yu., Ulanova G.I., Karbysheva S.B., Mironova R.I., Vahrameeva G.M., Gubareva T.I., Pavlov V.M., Dyatlov I.A. [Isolation of the Central Asian subspecies of the tularemia microbe in the Altai Territory]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2013; (1):66–9. DOI: 10.21055/0370-1069-2013-1-66-69.
6. Timofeev V., Bakhteeva I., Titareva G., Kopylov P., Christiany D., Mokrievich A., Dyatlov I., Vergnaud G. Russian isolates enlarge the known geographic diversity of Francisella tularensis subsp. mediasiatica. PLoS One. 2017; 12(9):e0183714. DOI: 10.1371/journal.pone.0183714.
7. Shevtsov A., Izbanova U., Amirgazin A., Kairzhanova A., Dauletov A., Kiyan V., Vergnaud G. Genetic homogeneity of Francisella tularensis subsp. mediasiatica strains in Kazakhstan. Pathogens. 2024; 13(7):581. DOI: 10.3390/pathogens13070581.
8. MLVAbank. Public Databases. (Cited 26 July 2024). [Internet]. Available from: https://microbesgenotyping.i2bc.paris-saclay.fr/databases/.
9. Sorokin V.M., Pavlovich N.V., Cimbalistova M.V., Vodop’yanov A.S., Pisanov R.V., Bazarova G.H.,., Rozhdestvenskiy Е.N., Noskov A.K. [Genetic diversity of Francisella tularensis subspecies mediasiatica strains]. Vestnik Biotekhnologii i Fiziko-Khimicheskoi Biologii im. Yu.A. Ovchinnikova [Bulletin of Biotechnology and Physicochemical Biology named after Yu.A. Ovchinnikov]. 2023; 19(4):21–7.
10. Zhou Z., Alikhan N.F., Sergeant M.J., Luhmann N., Vaz C., Francisco A.P., Carriço J.A., Achtman M. GrapeTree: visualization of core genomic relationships among 100,000 bacterial pathogens. Genome Res. 2018; 28(9):1395–404. DOI: 10.1101/gr.232397.117.
11. Sorokin V.M., Vodopyanov A.S., Tsimbalistova M.V., Pavlovich N.V. [Differentiation of the Francsella tularensis subspecies by the INDEL typing method]. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii [Journal of Microbiology, Epidemiology and Immunobiology]. 2022; 99(2):193–202. DOI: 10.36233/0372-9311-189.
12. Sorokin V.M., Vodopyanov A.S., Pavlovich N.V., Tsimbalistova M.V. [Method for determining Francisella tularensis subspecies using multi-primer PCR]. RF Patent No. 2765495, publ. 31 Jan 2022.
13. Hasle G., Bjune G., Edvardsen E., Jakobsen C., Linnehol B., Røer J.E., Mehl R., Røed K.H., Pedersen J., Leinaas H.P. Transport of ticks by migratory passerine birds to Norway. J. Parasitol. 2009; 95(6):1342–51. DOI: 10.1645/GE-2146.1.
14. Pitó A., Bukor B., Győrig E., Brlík V., Kontschán J., Keve G., Takács N., Hornok S. Investigations of the tick burden on passeriform, water-associated and predatory birds reveal new tick-host associations and habitat-related factors of tick infestation. Parasit. Vectors. 2024; 17(1):144. DOI: 10.1186/s13071-024-06229-1.
15. Padeshki P.I., Ivanov I.N., Popov B., Kantardjiev T.V. The role of birds in dissemination of Francisella tularensis: first direct molecular evidence for bird-to-human transmission. Epidemiol. Infect. 2010; 138(3):376–9. DOI: 10.1017/S0950268809990513.
16. Lopes de Carvalho I., Zé-Zé L., Alves A.S., Pardal S., Lopes R.J., Mendes L., Núncio M.S. Borrelia garinii and Francisella tularensis subsp. holarctica detected in migratory shorebirds in Portugal. Eur. J. Wildl. Res. 2012; 58(5):857–61. DOI: 10.1007/s10344-012-0617-3.
Review
For citations:
Sorokin V.M., Pavlovich N.V., Tsimbalistova M.V., Aronova N.V., Vodop’yanov A.S., Pisanov R.V. Genetic Heterogeneity of Francisella tularensis Subspecies mediasiatica Strains. Problems of Particularly Dangerous Infections. 2025;(3):154-159. (In Russ.) https://doi.org/10.21055/0370-1069-2025-3-154-159
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