Analysis of Malfunctions of Microbiological Safety Cabinets and Ventilation Systems in Bacteriological Laboratories
https://doi.org/10.21055/0370-1069-2025-3-186-189
Abstract
The aim of the work was to identify and analyze failures of microbiological safety cabinets and supply and exhaust ventilation systems in bacteriological laboratories.
Materials and methods. The technical condition and integrity of the HEPA filters of the microbiological safety cabinets and air purification filters for supply and exhaust ventilation were checked in accordance with the requirements of articles 188–191 of SanPiN 3.3686-21, GOST R EN 12469-2010. Over the period of 2018–2023, 926 studies were conducted to verify the protective effectiveness of microbiological safety cabinets and air purification filters; of those, microbiological safety cabinets class 1, 2, 3 – 524 units, filters for air purification of supply and exhaust ventilation – 402. The inspection was performed using the following bits of equipment (measuring instruments): Solair 3100 portable particle counter, Atomizer Aero Generator ATM 226 test aerosol generator, Dilution System DIL 554 aerosol diluent, Testo 510 differential pressure gauge.
Results and discussion. As a result of the study conducted, it was established that out of 524 cabinets, 488 units (93 %) met the requirements of sanitary regulations and were approved for further use. 36 microbiological safety cabinets did not pass the test for compliance with the requirements of sanitary rules and regulations by the following parameters: the incoming flow rate – 4 units, the downward flow rate – 6 units, and the protective efficiency of the filter – 26 units. Of the 26 cases of the protective effectiveness violation of the air purification filters in the microbiological safety cabinets, 15 filters had their integrity compromised at one or 2–3 surface points, and 6 pieces of equipment had diffusely damaged entire filter surface. 5 pieces of equipment had a leak of test aerosol around the perimeter of the installation and breach of the sealing of the filter in the housing of the cabinet.
About the Authors
E. A. Snatenkov
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
7, Golubinskaya St., Volgograd, 400131
A. A. Kovalenko
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
7, Golubinskaya St., Volgograd, 400131
K. A. Rotov
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
7, Golubinskaya St., Volgograd, 400131
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Review
For citations:
Snatenkov E.A., Kovalenko A.A., Rotov K.A. Analysis of Malfunctions of Microbiological Safety Cabinets and Ventilation Systems in Bacteriological Laboratories. Problems of Particularly Dangerous Infections. 2025;(3):186-189. (In Russ.) https://doi.org/10.21055/0370-1069-2025-3-186-189
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