Studies of the Composition of the Cell Antigen Preparations Obtained from Pathogenic Burkholderia, Using Immunoblotting
https://doi.org/10.21055/0370-1069-2015-2-46-49
Abstract
Carried out has been comparative analysis of the cell antigen immune-electrophoregrammes of B. pseudomallei C-141, B. mallei 10230, B. thailandensis 264, and B. cepacia 25416 after immunoblotting with immune rabbit sera to living cells of avirulent B. pseudomallei 107, celllular and extracellular antigens of B. pseudomallei 107, B. pseudomallei C-141, B. mallei 10230, B. thailandensis 264, and B. cepacia 25416. Visual investigation has revealed the presence of fractions with molar mass of 18.4 and 35 kDa in B. malei 10230 cell antigens’ electrophoregramme obtained by means of immunoblotting with serum to B. malei 10230 cell antigens, as distinct from B. pseudomallei C-141 electrophoregramme with the same serum. This makes it possible to distinguish the pathogenic Burkholderia. At the same time utilization of heterologous immune sera enhances discriminating capacity of the comparative assay. The level of reproducibility of immune-electrophoregrammes allows for the deployment of the similarity coefficient for computer-based comparative analysis.
About the Authors
A. A. Budchenko
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
I. Yu. Mazurova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
V. I. Ilyukhin
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
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Review
For citations:
Budchenko A.A., Mazurova I.Yu., Ilyukhin V.I. Studies of the Composition of the Cell Antigen Preparations Obtained from Pathogenic Burkholderia, Using Immunoblotting. Problems of Particularly Dangerous Infections. 2015;(2):46-49. (In Russ.) https://doi.org/10.21055/0370-1069-2015-2-46-49
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