Management of the Investigation and Detection of Legionellosis Agent in the Environmental Samples during the Preparation and Holding of the XXII Winter Olympics and XI Paralympics in Sochi

E. B. Ezhlova; Yu. V. Demina; A. N. Kulichenko; S. A. Portenko; A. S. Gus’Kov; E. S. Pochtareva; I. V. Savel’Eva; A. S. Volynkina; V. N. Savel’Ev; M. E. Mikhailova; I. V. Kuznetsova; O. A. Bobenko; D. V. Efremenko; E. S. Kazakova; T. Yu. Krasovskaya; V. E. Kuklev; I. A. Kas’Yan; E. A. Bil’Ko; E. V. Mitsevich; I. P. Mitsevich; M. E. Platonov; M. G. Teimurazov; O. V. Polosenko; V. E. Eldinova; E. A. Boiko; V. I. Malay; V. P. Klindukhov; T. V. Grechanaya; P. N. Nikolaevich; V. A. Biryukov; I. I. Bozhko; L. I. Shcherbina; O. A. Pogudina

doi:10.21055/0370-1069-2015-2-50-53

Management of the Investigation and Detection of Legionellosis Agent in the Environmental Samples during the Preparation and Holding of the XXII Winter Olympics and XI Paralympics in Sochi

E. B. Ezhlova, Yu. V. Demina, A. N. Kulichenko, S. A. Portenko, A. S. Gus’Kov, E. S. Pochtareva, I. V. Savel’Eva, A. S. Volynkina, V. N. Savel’Ev, M. E. Mikhailova, I. V. Kuznetsova, O. A. Bobenko, D. V. Efremenko, E. S. Kazakova, T. Yu. Krasovskaya, V. E. Kuklev, I. A. Kas’Yan, E. A. Bil’Ko, E. V. Mitsevich, I. P. Mitsevich, M. E. Platonov, M. G. Teimurazov, O. V. Polosenko, V. E. Eldinova, E. A. Boiko, V. I. Malay, V. P. Klindukhov, T. V. Grechanaya, P. N. Nikolaevich, V. A. Biryukov, I. I. Bozhko, L. I. Shcherbina, O. A. Pogudina

https://doi.org/10.21055/0370-1069-2015-2-50-53

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Abstract

Provided are the data on the management and carrying out of the laboratory control over the hot-water supply systems of the sports venues and accommodation areas for the presence of Legionella pneumophila during the XXII Olympics and XI Paralympics in Sochi, 2014. Inspected have been 105 facilities. The samples from 37 of them showed positive. L. pneumophila DNA concentration varies between 2.19·10² and 3.92·10⁷ genome equivalents (g.e.)/l. By means of bacteriological investigation detected have been legionella colonies in the water supply systems of 16 facilities, at one of the items - the loading is over 1·10⁴ colony-forming units/l. Performed has been comparative analysis of the results obtained using PCR assay and bacteriological test. Based on the genotyping of 7 isolated strains serogroup 1 and 2 strains of 2-14 serogroups it is concluded that L. pneumophila strains circulating in the resort town Sochi are genetically heterogeneous.

Keywords

Олимпийские игры, легионеллез, Legionella pneumophila, микробиологический мониторинг, полимеразная цепная реакция, бактериологический метод, генотипирование, Olympic Games, legionellosis, microbiological survey, polymerase chain reaction, bacteriological test, genotyping

About the Authors

E. B. Ezhlova

Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Russian Federation

Yu. V. Demina

Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Russian Federation

A. N. Kulichenko

Stavropol Research Anti-Plague Institute
Russian Federation

S. A. Portenko

Russian Research Anti-Plague Institute “Microbe”
Russian Federation

A. S. Gus’Kov

Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Russian Federation

E. S. Pochtareva

Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Russian Federation

I. V. Savel’Eva

Stavropol Research Anti-Plague Institute
Russian Federation

A. S. Volynkina

Stavropol Research Anti-Plague Institute
Russian Federation

V. N. Savel’Ev

Stavropol Research Anti-Plague Institute
Russian Federation

M. E. Mikhailova

Stavropol Research Anti-Plague Institute
Russian Federation

I. V. Kuznetsova

Stavropol Research Anti-Plague Institute
Russian Federation

O. A. Bobenko

Stavropol Research Anti-Plague Institute
Russian Federation

D. V. Efremenko

Stavropol Research Anti-Plague Institute
Russian Federation

E. S. Kazakova

Russian Research Anti-Plague Institute “Microbe”
Russian Federation

T. Yu. Krasovskaya

Russian Research Anti-Plague Institute “Microbe”
Russian Federation

V. E. Kuklev

Russian Research Anti-Plague Institute “Microbe”
Russian Federation

I. A. Kas’Yan

Russian Research Anti-Plague Institute “Microbe”
Russian Federation

E. A. Bil’Ko

Russian Research Anti-Plague Institute “Microbe”
Russian Federation

E. V. Mitsevich

State Research Center for Applied Microbiology and Biotechnology
Russian Federation

I. P. Mitsevich

State Research Center for Applied Microbiology and Biotechnology
Russian Federation

M. E. Platonov

State Research Center for Applied Microbiology and Biotechnology
Russian Federation

M. G. Teimurazov

State Research Center for Applied Microbiology and Biotechnology
Russian Federation

O. V. Polosenko

State Research Center for Applied Microbiology and Biotechnology
Russian Federation

V. E. Eldinova

Black Sea Plague Control Station
Russian Federation

E. A. Boiko

Black Sea Plague Control Station
Russian Federation

V. I. Malay

Black Sea Plague Control Station
Russian Federation

V. P. Klindukhov

Rospotrebnadzor Administration in the Krasnodar Territory
Russian Federation

T. V. Grechanaya

Rospotrebnadzor Administration in the Krasnodar Territory
Russian Federation

P. N. Nikolaevich

Rospotrebnadzor Administration in the Krasnodar Territory
Russian Federation

V. A. Biryukov

Rospotrebnadzor Administration in the Krasnodar Territory
Russian Federation

I. I. Bozhko

Rospotrebnadzor Administration in the Krasnodar Territory
Russian Federation

L. I. Shcherbina

Center of Hygiene and Epidemiology in the Krasnodar Territory
Russian Federation

O. A. Pogudina

Territorial Subdivision of the Rospotrebnadzor Administration in the Krasnodar Territory in the Resort Town of Sochi
Russian Federation

References

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3. Kao P.M., Tung M.C., Hsu B.M., Chiu Y.C., She C.Y., Shen S.M., Huang Y.L., Huang W.C. Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay. Environ. Sci. Pollut. Res. Int. 2013; 20(9):6128–37.

4. Bonetta Sa., Bonetta Si., Ferretti E., Balocco F., Carraro E. Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and culture methods. J. Appl. Microbiol. 2010; 108(5):1576–83.

5. Yáñez M.A., Nocker A., Soria-Soria E., Múrtula R., Martínez L., Catalán V. Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. J. Microbiol. Methods. 2011; 85(2):124–30.

Review

For citations:

Ezhlova E.B., Demina Yu.V., Kulichenko A.N., Portenko S.A., Gus’Kov A.S., Pochtareva E.S., Savel’Eva I.V., Volynkina A.S., Savel’Ev V.N., Mikhailova M.E., Kuznetsova I.V., Bobenko O.A., Efremenko D.V., Kazakova E.S., Krasovskaya T.Yu., Kuklev V.E., Kas’Yan I.A., Bil’Ko E.A., Mitsevich E.V., Mitsevich I.P., Platonov M.E., Teimurazov M.G., Polosenko O.V., Eldinova V.E., Boiko E.A., Malay V.I., Klindukhov V.P., Grechanaya T.V., Nikolaevich P.N., Biryukov V.A., Bozhko I.I., Shcherbina L.I., Pogudina O.A. Management of the Investigation and Detection of Legionellosis Agent in the Environmental Samples during the Preparation and Holding of the XXII Winter Olympics and XI Paralympics in Sochi. Problems of Particularly Dangerous Infections. 2015;(2):50-53. (In Russ.) https://doi.org/10.21055/0370-1069-2015-2-50-53

This work is licensed under a Creative Commons Attribution 4.0 License.

ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)

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Problems of Particularly Dangerous Infections

Management of the Investigation and Detection of Legionellosis Agent in the Environmental Samples during the Preparation and Holding of the XXII Winter Olympics and XI Paralympics in Sochi

Full Text:

Abstract

Keywords

About the Authors

References

Review

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