Structural-Functional Analysis of Cryptic Plasmids in Yersinia pestis Strains from Two Natural Plague Foci of Russia
https://doi.org/10.21055/0370-1069-2015-4-82-85
Abstract
Identified are the complete nucleotide sequences of two cryptic plasmids - pCKF from the Central-Caucasian high-mountain plague focus and pTP33 - from Tuva mountain plague focus in Russia. It is established that the size of the pCKF is 5.4 kbp, and its G-C pair composition accounts for 38.4 %. The plasmid contains 8 open reading frames, encoding transport and secretion functions, in particular, secretion systems of the VI type. The size of another cryptic plasmid, pTP33, is 33.8 kbp, and G-C pair composition accounts for 50.3 %. It contains 52 open reading frames, the majority of which have been classified as phage proteins. Hence, it is inferred that pTP33 is a ring genome of phage. It also has two genes of two-component protein system, toxin-antitoxin, YoeB/YefM, that affects replication apparatus of bacteria.
About the Authors
E. G. Oglodin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
G. N. Odinokov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. P. Guseva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Bugorkova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
V. V. Kutyrev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Oglodin E.G., Eroshenko G.A., Kukleva L.M., Odinokov G.N., Guseva N.P., Bugorkova S.A., Kutyrev V.V. Structural-Functional Analysis of Cryptic Plasmids in Yersinia pestis Strains from Two Natural Plague Foci of Russia. Problems of Particularly Dangerous Infections. 2015;(4):82-85. (In Russ.) https://doi.org/10.21055/0370-1069-2015-4-82-85
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