Development of Algorithm for Identification of Brucellosis Agent Cultures Using MALDI-TOF Mass-Spectrometry
https://doi.org/10.21055/0370-1069-2015-4-96-99
Abstract
Given are the results of direct protein profiling of brucella strain collection using matrix laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS). Standardized is the method for brucellosis agent culture disinfection and subsequent assessment. Obtained are 59 representative protein profiles of six major species ( B. melitensis, B. abortus, B. canis, B. ovis, B. neotomae ), which are included into electronic database of mass-spectra, integrated in Biotyper v 3.0 software (Bruker Daltonics, Germany). Analysis of mass-spectra of brucella strains under investigation revealed 17 genus-specific fragments within the mass range of 2000-20000 Da, the combination of which can be used for Brucella spp . identification. In addition, described are the fragments, specific for certain brucella species and strains, promising as markers for intraspecific differentiation. Based on the data received developed is a standardized algorithm of identification and differentiation of brucellosis agent cultures applying MALDI-TOF mass spectrometry.
About the Authors
D. V. Ul’Shina
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
D. A. Kovalev
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
O. V. Bobrysheva
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
G. I. Lyamkin
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
A. A. Khudoleev
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
Yu. V. Siritsa
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
A. N. Kulichenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
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Review
For citations:
Ul’Shina D.V., Kovalev D.A., Bobrysheva O.V., Lyamkin G.I., Khudoleev A.A., Siritsa Yu.V., Kulichenko A.N. Development of Algorithm for Identification of Brucellosis Agent Cultures Using MALDI-TOF Mass-Spectrometry. Problems of Particularly Dangerous Infections. 2015;(4):96-99. (In Russ.) https://doi.org/10.21055/0370-1069-2015-4-96-99
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