Improvement of Technology of Cholera Toxin B-Subunit Production
https://doi.org/10.21055/0370-1069-2015-4-100-102
Abstract
Consideration is given to implementation of state-of-the-art filtration technologies for up-scaled manufacturing of cholera toxin B-subunit, produced by recombinant Vibrio cholerae non O1 KM93 strain. Selected are micro- and ultra-filtration membranes to be incorporated into manufacturing method. Investigated are the properties of cholera toxin B-subunit, obtained applying the pilot technology. The engineered method for up-scaled manufacturing of cholera toxin B-subunit makes the procedure easier-to-maintain due to tangential micro- and ultra-filtration, performed at the stage of purification and concentration. It excludes labor-consuming chromatographic purification, while retaining B-subunit properties. The studies undertaken make it possible to manufacture cholera toxin B-subunit with the same characteristics as in the case of the pilot technology, but under production conditions, and use it as a component for chemical cholera vaccine.
About the Authors
A. V. Komissarov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Eremin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. V. Gromova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
Yu. A. Aleshina
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
E. M. Kuznetsova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. G. Avdeeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. F. Livanova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. K. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Komissarov A.V., Eremin S.A., Volokh O.A., Gromova O.V., Aleshina Yu.A., Kuznetsova E.M., Avdeeva N.G., Livanova L.F., Nikiforov A.K. Improvement of Technology of Cholera Toxin B-Subunit Production. Problems of Particularly Dangerous Infections. 2015;(4):100-102. (In Russ.) https://doi.org/10.21055/0370-1069-2015-4-100-102
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