Development of Multi-Analytical Immune-Chromatographic Device for Toxin Indication
https://doi.org/10.21055/0370-1069-2015-4-103-106
Abstract
Objective of the work was to construct immune-chromatographic device for indication of five toxin types in a single processing cycle and investigation of its capacities regarding environment sample analysis and food testing. Obtained were the conjugates of monoclonal antibodies with nano-particles of colloid gold, formed were membrane composites for sandwich immune-chromatography of separate toxins. On the basis of distinct test panels designed was immune-chromatographic device for multiple analyses, which contained multi-analytical detection element, swab for surface sampling, and storage vessel with test buffer. This device allowed for concurrent identification of botulinic toxins A, B types concentrated up to 5 and 10 ng/ml, respectively; staphylococcal toxin B type - 40 ng/ml, cholera toxin - 500 ng/ml, and ricin - 80 ng/ml, within a single processing cycle. Demonstrated was the possibility of toxin detection in washings from ambient object surfaces, as well as in foodstuffs. Described was preliminary food sample preparation procedure.
About the Authors
I. V. Shilenko
State Research Institute of Biological Engineering
Russian Federation
Russian Federation
S. P. Yarkov
State Research Institute of Biological Engineering
Russian Federation
Russian Federation
E. K. Shaulina
State Research Institute of Biological Engineering
Russian Federation
Russian Federation
A. A. Titov
State Research Institute of Biological Engineering
Russian Federation
Russian Federation
A. N. Brovkina
State Research Institute of Biological Engineering
Russian Federation
Russian Federation
E. N. Khramov
State Research Institute of Biological Engineering
Russian Federation
Russian Federation
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Review
For citations:
Shilenko I.V., Yarkov S.P., Shaulina E.K., Titov A.A., Brovkina A.N., Khramov E.N. Development of Multi-Analytical Immune-Chromatographic Device for Toxin Indication. Problems of Particularly Dangerous Infections. 2015;(4):103-106. (In Russ.) https://doi.org/10.21055/0370-1069-2015-4-103-106
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