Studies of Freeze-Drying of Cholera Chemical Vaccine Immunogens
https://doi.org/10.21055/0370-1069-2016-1-90-93
Abstract
Objective of the study was to substantiate experimentally the possibility to enhance efficiency of manufacturing process through application of novel engineering solutions in the course of freeze-drying of vaccine immunogens. Materials and methods. Choleragen-anatoxin and O-antigens, Inaba and Ogawa, served as specific immunogenic components of the vaccine. Identified were temperature values for O-antigen eutectics (approx. -35 ºC). At reaching -55 ºC choleragen-anatoxin froze incompletely (fractionally). Full solidification of the preparation was achieved by means of the “annealing”. Revealed was non-effect of the tested freeze modes on the properties of antigen components of chemical cholera vaccine. Desired values of technological parameters for primary drying and desorption were specified by trial. Investigations of activity of chemical cholera vaccine immunogen lyophilizates testified to the compliance with the rated critical requirements. Results and conclusions. Results of the study provided for significant time expenditure reduction and obtainment of high-quality preparations at once.
About the Authors
A. V. Komissarov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. N. Kochkalova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. V. Sinitsyna
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Badarin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. I. Kostyleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. D. Klokova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. K. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Komissarov A.V., Kochkalova N.N., Sinitsyna N.V., Badarin S.A., Kostyleva N.I., Volokh O.A., Klokova O.D., Nikiforov A.K. Studies of Freeze-Drying of Cholera Chemical Vaccine Immunogens. Problems of Particularly Dangerous Infections. 2016;(1):90-93. (In Russ.) https://doi.org/10.21055/0370-1069-2016-1-90-93
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