Method of Accelerated Evaluation of Antimicrobial Preparation Efficacy against Experimental Brucellosis
https://doi.org/10.21055/0370-1069-2016-2-79-82
Abstract
Objective of the study was to elaborate the method for preliminary accelerated evaluation of antimicrobial preparation efficacy in case of in vivo brucellosis. Materials and methods. Outbred BALB/c mice with the acute septic experimental brucellosis, caused by intravenous inoculation with sub-lethal doses of the agent, served as biological models. Concurrently with the challenge, animals were given antimicrobial treatment. They took preparations of different types. Further, against the background of antimicrobial therapy, changes of quantitative microbe content in the internal organs of animals were studied, applying bacteriological method. Based on the data obtained index of antimicrobial activity was calculated, according to the value of which, it is possible to rank the drugs under study. Results and discussion. Results of preparation potency estimation, received with the help of the developed method, and results obtained after complete standard regimens show high-level correlation dependence (correlation ratio being 0.73), which makes the method suitable for screening of new antimicrobials in reference to brucellosis agent.
About the Authors
V. Yu. Okhapkina
Affiliated Branch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
N. V. Pyatkova
Affiliated Branch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
A. K. Fedotov
Affiliated Branch of the 48th Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
References
1. Bunin K.V., Belov G.F. [Immune Antibiotic Treatment of Recurrent and Chronic Forms of Infectious Diseases]. Novosibirsk: Nauka; 1982. 280 p.
2. Lobzin Yu.V., editor. [Infectious Diseases: Textbook for Medical College Students]. St. Petersburg: Foliant; 2001. 542 p.
3. Lobzin Yu.V., editor.[Guidelines on Infectious Diseases]. St. Petersburg: Foliant; 2000. 460 p.
4. Maletskaya O.V. [Potency of certain novel antibiotics in case of experimental brucellosis treatment]. Antibiot. Khimioterapiya. 2002; 47(11): 4–7.
5. Maletskaya O.V. [Novel approaches to treatment of brucellosis]. Epidemiol. Infek. Bol. 2003; 6:32–5.
6. Elberg S.S. Guide to the diagnosis, treatment and prevention of human brucellosis. Geneva: WHO; 1985. Р. 345–54.
7. Farreli L.D., Robertson L.R. The treatment of brucellosis. J. Chemother. 1980; 6:695–97.
8. Hall W.H. Modern chemotherapy for brucellosis in humans. Rev. Infect. Dis. 1990; 12(6):1060–99.
9. Malik G.M. Early clinical response to different therapeutic regimens for human brucellosis Am. J. Trop. Med. Hyg. 1998; 58(2):190–91.
10. Shehabi A., Shakir K. Diagnosis and treatment of 106 cases of human brucellosis. J. Infect. 1990; 20(1):5–10. DOI: 10.1016/S0163-4453(90)92214-6.
11. Solera J. Treatment of human brucellosis J. Med. Liban. 2000; 48(4):255–63.
Review
For citations:
Okhapkina V.Yu., Pyatkova N.V., Fedotov A.K. Method of Accelerated Evaluation of Antimicrobial Preparation Efficacy against Experimental Brucellosis. Problems of Particularly Dangerous Infections. 2016;(2):79-82. (In Russ.) https://doi.org/10.21055/0370-1069-2016-2-79-82
Views:
830
Email this article 