Preparation of Hyperimmune Sera to Thermoextracts of Brucella S- and L-Forms
https://doi.org/10.21055/0370-1069-2016-4-98-101
Abstract
Objective of the study was to prepare hyperimmune sera to Brucella S- and L-form thermo-extracts. Materials and methods. Thermo-extracts from Brucella abortus I-206 strain in S- and L-forms were used. Chinchilla rabbits were used as animals, sera producers. Specific activity was determined by test-tube agglutination with Brucella L-form corpuscular experimental and commercial Brucella colored diagnosticums, while specificity – with heterologous strains: Francisella tularensis 15 NIIEG, Vibrio choleraе 5 (Ogawa), V. choleraе 35 А3 (Inaba), Yersinia enterocolitica О:9, Y. pestis EV NIIEG, Escherichia coli 3912/41. Protein content in the obtained extract was defined using Lowry method. Results and conclusions. Consequently, the antigen activity of the generated thermo-extracts has been shown, the immunization schemes have been developed permitting to obtain highly active and specific sera that could be used for identification and differentiation of brucellosis causative agent.
About the Authors
L. M. Мikhailov
Irkutsk Research Anti-Plague Institute, Irkutsk
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
N. M. Andreevskaya
Irkutsk Research Anti-Plague Institute, Irkutsk
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
V. A. Мikhailova
Irkutsk Research Anti-Plague Institute, Irkutsk
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
N. L. Barannikova
Irkutsk Research Anti-Plague Institute, Irkutsk
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
L. E. Tokareva
Irkutsk Research Anti-Plague Institute, Irkutsk
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
K. Yu. Yastremskaya
Irkutsk Research Anti-Plague Institute, Irkutsk
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
S. V. Balakhonov
Irkutsk Research Anti-Plague Institute, Irkutsk
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
References
1. Mikhailov L.M., Kalinovsky A.I., Andreevskaya N.M., Mikhailova V.A., Repina L.P. [Method for the production of diagnostic agglutinating se- rum against Brucella L-form]. RF Patent No 2242765, 20.12.2004.
2. Mikhailov L.M., Kalinovsky A.I., Andreevskaya N.M., Mikhailova V.A., Balakhonov S.V., Shestopalov M.Yu., Tatarnikova O.G., Repina L.P., Boldina A.A., Kapustin Yu.M. [Anti-sera to Brucella L-from]. Bulletin of the East-Siberian Scientific Center, RAMS Siberian Branch. 2004; 1(2):143–6.
3. Mikhailov L.M., Kalinovsky A.I., Barannikova N.L., Balakhonov S.V., Markov E.Yu., Nikolaev V.B., Kozulina K.Yu., Shestopalov M.Yu., Andreevskaya N.M., Mikhailova V.A., Tatarnikova O.G., Kuznetsov V.I. [Method for the production of antigen preparation from Brucella L-form]. RF Patent No 2416429, 20.04. 2011.
4. Onishchenko G.G., Kutyrev V.V., editors. [Laboratory Diagnostics of Dangerous Infectious Diseases. Practice Guidelines]. 2nd Edition, M.: CJSC “Shiko”; 2013. 560 p.
5. Sayapina L.V., Komratov A.V., Khramov M.V., Toropchin M.I., Fadeykina O.V., Volkova R.A., Rusanova D.V., Nemirovskaya T.I., Lyamkina G.I. [Certification of the departmental standard specimen of diagnostic polyvalent Brucella serum for agglutination assay]. Biopreparaty. Profilakt. Diagnost. Lechenie. 2014; 4(52):42–7.
6. Spirin A.S. [Spectrophotometric evaluation of the aggregate nucleic acid amount]. Biokhimiya. 1958; 23(5): 656–62.
7. Hanson R. [Chemical composition of bacterial cell]. In: [Methods of General Bacteriology]. Vol. 2. M.; 1984. P. 283–373.
8. Berman D.T., Kurtz R.S. Relationship of biological activities to struc- tures of Brucella abortus endotoxin and LPS. Ann. Inst. Pasteur Microbiol. 1987; 138(1):98–101.
9. Chenais, E. Bagge, E. Lambertz, S.T. Artursson, K. Yersinia entero- colitica serotype O:9 cultured from Swedish sheep showing serologically false-positive reactions for Brucella melitensis. Infect. Ecol. Epidemiol. 2012; 2:19027. DOI:10.3402/iee.v2i0.19027.
10. Kubler-Kielb J., Vinogradov E. Reinvestigation of the structure of Brucella O-antigens. Carbohydr. Res. 2013; 378:144–7.
11. Kubler-Kielb J., Vinogradov E. The study of the core part and non-repeating elements of the O-antigen of Brucella lipopolysaccharide. Carbohydr. Res. 2013; 366:33–7.
12. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein meas- urement with the Folin phenol reagent. J. Biol. Chem. 1951; 193(1):265–75.
Review
For citations:
Мikhailov L.M., Andreevskaya N.M., Мikhailova V.A., Barannikova N.L., Tokareva L.E., Yastremskaya K.Yu., Balakhonov S.V. Preparation of Hyperimmune Sera to Thermoextracts of Brucella S- and L-Forms. Problems of Particularly Dangerous Infections. 2016;(4):98-101. (In Russ.) https://doi.org/10.21055/0370-1069-2016-4-98-101
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