Properties of Experimental and Standard Preparations of the Protective O Antigen of Vibrio cholerae M41 Ogawa
https://doi.org/10.21055/0370-1069-2013-2-70-72
Abstract
Investigated are immunochemical, chemical and biochemical properties of the O-antigen preparations obtained from
Vibrio cholerae M41 classical biovar, serotype Ogawa strain using standard manufacturing and improved technologies of their concentrating. The preparations have been concentrated by ultrafiltration in the mode of tangential liquid flow with membranes of various cut-off levels for a particular molar weight. ELISA has revealed equal O-antigen load in all of these preparations. Studies of specific fractures by means of chromatographic and electrophoretic methods have demonstrated their properties to be alike. Chemical composition analysis has also identified coincidence in the load of components under specification. Thus, it has been proved that application of the improved
V. cholerae protective antigen concentrating technology for manufacturing cholera chemical bivalent palletized vaccine is justified.
Vibrio cholerae M41 classical biovar, serotype Ogawa strain using standard manufacturing and improved technologies of their concentrating. The preparations have been concentrated by ultrafiltration in the mode of tangential liquid flow with membranes of various cut-off levels for a particular molar weight. ELISA has revealed equal O-antigen load in all of these preparations. Studies of specific fractures by means of chromatographic and electrophoretic methods have demonstrated their properties to be alike. Chemical composition analysis has also identified coincidence in the load of components under specification. Thus, it has been proved that application of the improved
V. cholerae protective antigen concentrating technology for manufacturing cholera chemical bivalent palletized vaccine is justified.
About the Authors
Yu. A. Aleshina
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
V. I. Pavlova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Komissarov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. V. Gromova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. K. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
M. N. Kireev
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Eremin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Gaeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
L. F. Livanova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
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Review
For citations:
Aleshina Yu.A., Pavlova V.I., Komissarov A.V., Gromova O.V., Nikiforov A.K., Kireev M.N., Eremin S.A., Volokh O.A., Gaeva A.V., Livanova L.F. Properties of Experimental and Standard Preparations of the Protective O Antigen of Vibrio cholerae M41 Ogawa. Problems of Particularly Dangerous Infections. 2013;(2):70-72. (In Russ.) https://doi.org/10.21055/0370-1069-2013-2-70-72
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