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Problems of Particularly Dangerous Infections

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BIOLOGICAL PROPERTIES AND MOLECULAR-GENETIC CHARACTERISTICS OF BACILLUS ANTHRACIS STRAINS, ISOLATED DURING THE OUTBREAK OF ANTHRAX IN THE YAMALO-NENETS AUTONOMOUS DISTRICT IN 2016

https://doi.org/10.21055/0370-1069-2017-1-94-99

Abstract

Objective of the study was to identify phenotypic properties and genetic peculiarities of Bacillus anthracis strains, isolated during the outbreak of anthrax in the territory of Yamal in 2016.

Materials and methods. Investigated were the strains of anthrax agent, applying basic and subsequent identification tests and canSNP-, MLVA-genotyping methods and whole genome sequencing.

Results and conclusions. The results showed the identity of the phenotypic properties, canSNPand MLVA25-genotypes, and profiles of whole genome-sequencing, regardless of the source of the strains isolation. Confirmed was a common source of human infection. Defined were phylogenetic interrelations of the tested strains and their position in global B. anthracis population. For the first time ever explored was variability of the gene pattern, associated with pathogenicity, and demonstrated – the efficiency of the proposed algorithm for genetic typing.

About the Authors

A. N. Kulichenko
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


E. I. Eremenko
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


A. G. Ryazanova
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


L. Yu. Aksenova
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


D. A. Kovalev
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


S. V. Pisarenko
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


N. G. Varfolomeeva
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


A. M. Zhirov
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


А. S. Volynkina
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


N. P. Buravtseva
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


T. M. Golovinskaya
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


E. A. Koteneva
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


O. I. Tsygankova
Stavropol Research Anti-Plague Institute
Россия
13-15, Sovetskaya St., Stavropol, 355035, e-mail: snipchi@mail.stv.ru


I. A. Dyatlov
State Research Center of Applied Microbiology and Biotechnology
Россия
Obolensk, Moscow Region, 142279, Russian Federation, e-mail: info@obolensk.org


V. S. Timofeev
State Research Center of Applied Microbiology and Biotechnology
Россия
Obolensk, Moscow Region, 142279, Russian Federation, e-mail: info@obolensk.org


A. G. Bogun
State Research Center of Applied Microbiology and Biotechnology
Россия
Obolensk, Moscow Region, 142279, Russian Federation, e-mail: info@obolensk.org


I. V. Bakhteeva
State Research Center of Applied Microbiology and Biotechnology
Россия
Obolensk, Moscow Region, 142279, Russian Federation, e-mail: info@obolensk.org


A. A. Kislichkina
State Research Center of Applied Microbiology and Biotechnology
Россия
Obolensk, Moscow Region, 142279, Russian Federation, e-mail: info@obolensk.org


R. I. Mironova
State Research Center of Applied Microbiology and Biotechnology
Россия
Obolensk, Moscow Region, 142279, Russian Federation, e-mail: info@obolensk.org


G. M. Titareva
State Research Center of Applied Microbiology and Biotechnology
Россия
Obolensk, Moscow Region, 142279, Russian Federation, e-mail: info@obolensk.org


Yu. P. Skryabin
State Research Center of Applied Microbiology and Biotechnology
Россия
Obolensk, Moscow Region, 142279, Russian Federation, e-mail: info@obolensk.org


Yu. O. Selyaninov
National Research Institute of Veterinary Virology and Microbiology of the Russian Academy of Agricultural Sciences
Россия
1, Akademika Bakulova St., Volginsky, Vladimir Region, 601125, e-mail: VNIIVViM@niiv.petush.el-com.ru


I. Yu. Egorova
National Research Institute of Veterinary Virology and Microbiology of the Russian Academy of Agricultural Sciences
Россия
1, Akademika Bakulova St., Volginsky, Vladimir Region, 601125, e-mail: VNIIVViM@niiv.petush.el-com.ru


D. V. Kolbasov
National Research Institute of Veterinary Virology and Microbiology of the Russian Academy of Agricultural Sciences
Россия
1, Akademika Bakulova St., Volginsky, Vladimir Region, 601125, e-mail: VNIIVViM@niiv.petush.el-com.ru


References

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2. Lista F., Faggioni G., Valjevac S., Ciammaruconi A., Vaissaire J., Doujet C., Olivier G., De Santis R., Carattoli A., Ciervo A., Fasanella A., Orsini F., D'Amelio R., Pourcel C., Cassone A., Vergnaud G. Genotyping of Bacillus anthracis strains based on automated capillary 25-loci Multiple Locus Variable-Number Tandem Repeats Analysis. BMC Microbiol. 2006; 6:33. DOI: 10.1186/1471-2180-6-33.

3. Kenefic L.J., Beaudry J., Trim C., Huynh L., Zanecki S., Matthews M., Schupp J., Van Ert M., Keim P. A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis. J. Microbiol. Methods. 2008; 73(3):269-72. DOI: 10.1016/j.mimet.2007.11.014.


Review

For citations:

Kulichenko A.N., Eremenko E.I., Ryazanova A.G., Aksenova L.Yu., Kovalev D.A., Pisarenko S.V., Varfolomeeva N.G., Zhirov A.M., Volynkina А.S., Buravtseva N.P., Golovinskaya T.M., Koteneva E.A., Tsygankova O.I., Dyatlov I.A., Timofeev V.S., Bogun A.G., Bakhteeva I.V., Kislichkina A.A., Mironova R.I., Titareva G.M., Skryabin Yu.P., Selyaninov Yu.O., Egorova I.Yu., Kolbasov D.V. BIOLOGICAL PROPERTIES AND MOLECULAR-GENETIC CHARACTERISTICS OF BACILLUS ANTHRACIS STRAINS, ISOLATED DURING THE OUTBREAK OF ANTHRAX IN THE YAMALO-NENETS AUTONOMOUS DISTRICT IN 2016. Problems of Particularly Dangerous Infections. 2017;(1):94-99. https://doi.org/10.21055/0370-1069-2017-1-94-99

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