Experimental Preventive Anti-Tularemia Preparation
https://doi.org/10.21055/0370-1069-2013-2-73-77
Abstract
Designed is an experimental preparation of a prototype chemical tularemia vaccine (PCTV). It is composed of protective antigenic complex (PAC) of tularemia microbe and S-layer protein (Slp) of plague microbe. Determined is optimum ratio of these components in the preparation and schedule of its administration. Displayed are the results of its testing as regards physical-chemical properties, reactogenicity, specific activity and impact on the immune system of laboratory animals. It is found out that preparation of the prototype is non-toxic for white mice and Guinea pigs and has no damaging effect on their immune systems. Single-stage subcutaneous immunization with PCTV induces elaboration of high-level adaptive immunity in laboratory animals within 14–21 days: specific antibody generation and stimulation of immune system cell component. PCTV protective index for white mice in case of experimental tularemia, caused by
Francisella tularensis subsp. holarctica, is 87,5 % on average; in case of infecting with
F. tularensis subsp. nearctica – 50 %; and high-level immunity in both cases. High potency of the experimental preparation against tularemia caused by subsp. holarctica (protective index is 75 %) and high-grade immunity persistence is verified on the model of Guinea pigs too.
Francisella tularensis subsp. holarctica, is 87,5 % on average; in case of infecting with
F. tularensis subsp. nearctica – 50 %; and high-level immunity in both cases. High potency of the experimental preparation against tularemia caused by subsp. holarctica (protective index is 75 %) and high-grade immunity persistence is verified on the model of Guinea pigs too.
About the Authors
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
E. M. Kuznetsova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
E. A. Smol’Kova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
T. N. Shchukovskaya
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. A. Bugorkova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
N. G. Avdeeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
D. G. Filimonova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
T. P. Shmel’Kova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
S. N. Klyueva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. K. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
References
1. Volokh O.A., Shepelev I.A., Firstova V.V., Khramkova (Kuznetsova) E.M., Avdeeva N.G., Samokhvalova Yu.I., Eremin S.A., Dyatlov I.A. [Evaluation of immunobiological activity of tularemia microbe C-complex preparations as a prospective component of chemical vaccines]. Zh. Mikrobiol. Epidemol. Immunobiol. 2007; 3:16–21.
2. Dyatlov I.A., Volokh O.A. [Construction of plague microbe S-layer and possible ways of its application]. Biotekhnologia. 2004; 1:20–5.
3. Kuznetsova E.M., Volokh O.A., Smol`kova E.A., Shchukovskaya T.N., Shepelev I.A., Avdeeva N.G., Kravtsov A.L., Nikiforov A.K. [Immunobiological properties of Francisella tularensis antigen complexes]. Probl. Osobo Opasn. Infek. 2011; (109):46–9.
4. Sarkisova D.S., Perova Yu.L., editors [Microscopy accessories]. M.: Meditsina; 1996. 543 p.
5. Khlebnikov V.S., Golovlev I.R., Kulevatsky D.P., Averin S.F., Pshirkov S.Yu., Tokhtamysheva N.V., Zhemchugov V.E., Safonova L.A., Gerasimov V.N., Chugunov A.M., Vetchinin S.S., Galaktionov V.G., Afanas’ev S.S. [Investigation of biochemical, antigenic and protective properties of tularemia agent outer membrane]. Mol. Gen. Mikrobiol. Virusol. 1991; 7:15–20.
6. Barry E.M., Cole L.E., Santiago A.E. Vaccines against tularemia. Hum. Vaccine. 2009; 5(12):832–8.
7. Conlan J.W. Tularemia vaccines: recent developments and remaining hurdles. Future Microbiol. 2011; 6(4):391–405
8. Gregory S. H., Chen W. H., Mott S., Palardy J.E., Parejo N.A., Heninger S., Anderson C.A., Artenstein A.W., Opal S.M., Cross A.S. Detoxified endotoxin vaccine (J5dLPS/OMP) protects mice against lethal respiratory challenge with Francisella tularensis SchuS4. Vaccine. 2010; 28(16):2908–15.
9. Jahn-Schmid B., Messneer P., Under F.M., Sleytr U.B., Scheiner O., Kraft D. Toward selective elicitation of TnI-controlled vaccination responses: vaccine application of bacteria surface layer proteins. J. Biotechnol. 1996; 44:225–31.
10. Jia Q., Lee B.Y., Bowen R., Dillon B.J., Som S.M., Horwitz M.A. A Francisella tularensis live vaccine strain (LVS) mutant with a deletion in capB, encoding a putative capsular biosynthesis protein, is significantly more attenuated than LVS yet induces potent protective immunity in mice against F. tularensis challenge. Infect. Immun. 2010; 78(10):4341–55.
11. Khlebnikov V.S., Golovliov I.R., Kulevatsky D.P., Tokhtamysheva N.V., Averin S.F., Zhemchugov V.E., Pchelintsev S.Y., Afanasiev S.S., Shcherbakov G.Y. Outer membranes of a lipopolysaccharide-protein complex (LPS-17 kDa protein) as chemical tularemia vaccines. FEMS Immunol. Med. Microbiol. 1996; 13:227–33.
12. Pechous R.D., McCarthy T.R., Zahrt T.C. Working toward the future: insights into Francisella tularensis pathogenesis and vaccine development. Microbiol. Mol. Biol. Rev. 2009; 73(4):684–711.
13. Tarnvik A., Bergland L. Tularaemia. Eur. Respir. J. 2003; 21:361–73.
14. Under F.M., Messneer P., Smid B.J., Sleytr U.B. Vaccine application of crystalline bacterial surface layer proteins (S-layers). FEMS Microbiol. Rev. 1997; 20:157–8.
15. Zhemchugov V.E., Volokh O.A., Dyatlov I.A. et al. Outer membrane “C”-complex from F. tularensis vaccine strain 15 protect from challenge with F. tularensis type-A stran. In: The 4th International conference on tularemia. City of Bath (U.K.), 2003. Abstr. S-29.
Review
For citations:
Volokh O.A., Kuznetsova E.M., Smol’Kova E.A., Shchukovskaya T.N., Bugorkova S.A., Avdeeva N.G., Filimonova D.G., Shmel’Kova T.P., Klyueva S.N., Nikiforov A.K. Experimental Preventive Anti-Tularemia Preparation. Problems of Particularly Dangerous Infections. 2013;(2):73-77. (In Russ.) https://doi.org/10.21055/0370-1069-2013-2-73-77
Views:
1153
Email this article 