CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS
https://doi.org/10.21055/0370-1069-2017-2-32-35
Abstract
Objective of the study is to design the DNA-microarray for differentiation of Y. pestis strains of the main and non-main subspecies and biovars of the main subspecies. Materials and methods. Efficiency analysis for the devised means was conducted using 62 Y. pestis strains of various subspecies and biovars, isolated in the natural foci of Russia and neighboring countries. Results and conclusions. Selected have been the DNA-targets, probes and primers – calculated. Enhanced is the method of sub-specific and biovar differentiation of Y. pestis strains by means of DNA-microarray. DNA-chip with “Med24”, “glpD(-93)”, and “45” targets allows for prompt differentiation of the strains of the main and non-main subspecies and biovars of the main subspecies based on the presence and absence of fluorescent signal by the specific for the main subspecies and its biovars DNA-targets.
About the Authors
K. A. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
D. V. Utkin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
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Review
For citations:
Nikiforov K.A., Utkin D.V., Kukleva L.M., Eroshenko G.A. CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS. Problems of Particularly Dangerous Infections. 2017;(2):32-35. (In Russ.) https://doi.org/10.21055/0370-1069-2017-2-32-35
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