PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA
https://doi.org/10.21055/0370-1069-2017-2-78-80
Abstract
Objective of the study is to prepare rabbit monospecific anti-abortus serum to a Brucella S-form thermoextract, as well as to evaluate its activity and specificity. Materials and methods. Utilized was polyvalent hyperimmune serum obtained to Brucella thermoextract. It was adsorbed by a strain of heterologous species Brucella melitensis 16 N. Reference, vaccinal and field Brucella strains were used to estimate the activity and specificity of the serum produced. Results and conclusions. This serum possesses high activity (1:160, 1:320 titer) and specificity, and the results completely coincide with the data specified in the table of differential properties presented in МR 3.1.7.1189-03 “Prophylaxis and laboratory diagnostics of human brucellosis». The outlined method for manufacturing monospecific Brucella anti-abortus serum permits for a considerable decrease in biological hazard for personnel involved in the technological operations.
About the Authors
L. M. Мikhailov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
N. L. Barannikova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
L. E. Tokareva
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
N. M. Andreevskaya
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
V. A. Мikhailova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
V. I. Kuznetsov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
K. Yu. Yastremskaya
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
S. V. Balakhonov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
78, Trilissera St., Irkutsk, 664047
Russian Federation
78, Trilissera St., Irkutsk, 664047
References
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Review
For citations:
Мikhailov L.M., Barannikova N.L., Tokareva L.E., Andreevskaya N.M., Мikhailova V.A., Kuznetsov V.I., Yastremskaya K.Yu., Balakhonov S.V. PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA. Problems of Particularly Dangerous Infections. 2017;(2):78-80. (In Russ.) https://doi.org/10.21055/0370-1069-2017-2-78-80
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