Peculiarities of the Conjugative pSa Plasmid Transfer from Escherichia coli into Francisella tularensis
https://doi.org/10.21055/0370-1069-2013-2-80-82
Abstract
E. coli C600, while in tularemia microbe, this plasmid has only one shape. After the transfer of the plasmid from
E. coli cells into
F. tularensis cells each individual clone of tularemia microbe possesses a plasmid which differs in its electrophoretic mobility from plasmids of other clones.
About the Authors
A. N. MokrievichRussian Federation
N. A. Shishkova
Russian Federation
I. A. Dyatlov
Russian Federation
V. M. Pavlov
Russian Federation
References
1. Abaev I.V., Astashkin E.I., Pachkunov D.M., Stagis I.I., Shitov V.T., Svetoch E.A. [Pseudomonas mallei and Pseudomonas pseudomallei: introduction and maintenance of natural and recombinant plasmid replicons]. Mol. Genet. Mikrobiol. Virusol. 1995; 1:28–36.
2. Maniatis T., Frich E., Sembruk G. [Methods of Gene-Engineering. Molecular Cloning]. M.: Mir; 1984. 479 p.
3. Pomerantsev A.P., Domaradsly I.V., Shishkova N.A. [Hybrid pSKFT5 plasmid provides for Sa plasmid transfer from Escherichia coli cells into the vaccine strain of tularemia microbe]. Mol. Genet. Mikrobiol. Virusol. 1991, 8:13–6.
4. Farrand S.K., Kado C.I., Ireland C.R. Supression of tumoregenicity by the IncW R plasmid pSa in Agrobacterium tumefaciens. Mol. Gen. Genet. 1981; 181(1):44–51.
5. Ireland C.R. Detailed restriction enzyme map of crown gall-supressive IncW plasmid pSa, showing ends of deletion causing chloramphenicol sensitivity. J. Bacteriol. 1983; 155:722–7.
6. Pavlov V.M., Mokrievich A.N., Volkovoy K. Cryptic plasmid pFNL10 from Francisella novicida-like 6168: The base of plasmid vectors for Francisella tularensis. FEMS Immunol. Med. Microbiol. 1996; 13:253–6.
7. Rigby C.E., Fraser A.D. Plasmid transfer and plasmid-mediated genetic exchange in Brucella abortus. Can. J. Vet. Res. 1989; 53(3):326–30.
8. Watanabe T., Furuse C., Sakaizumi S. Transduction of various R factors by phage P1 in Escherichia coli and by phage P22 in Salmonella typhimurium. J. Bacteriol. 1968, 96:1791–5.
Review
For citations:
Mokrievich A.N., Shishkova N.A., Dyatlov I.A., Pavlov V.M. Peculiarities of the Conjugative pSa Plasmid Transfer from Escherichia coli into Francisella tularensis. Problems of Particularly Dangerous Infections. 2013;(2):80-82. (In Russ.) https://doi.org/10.21055/0370-1069-2013-2-80-82