Molecular-Genetic and Phenotypic Peculiarities of Plague Agent Strains Isolated in Vietnam
https://doi.org/10.21055/0370-1069-2017-4-45-49
Abstract
Objective of the study is to investigate phenotypic and molecular-genetic features and perform whole genome sequencing of Y. pestis strains isolated in Vietnam. Materials and methods. Studied were phenotypic and genotypic peculiarities of 20 plague agent strains isolated in different prefectures of Vietnam. Carried out was SNP-analysis of the strains, sequenced were genomes of 8 Y. pestis strains. Results and conclusions. Based on the results of studies of differential biochemical characteristics all the investigated strains were attributed to oriental biovar of the main subspecies of plague agent, which was confirmed by the presence of marker indel mutation – deletion of 93 bps in glpD gene. Investigated was also the plasmid composition of the strains. On the basis of the conducted genome sequencing and SNP-analysis appurtenance of 19 out of 20 strains under examination was determined. They belong to 1.ORI2v phylogenetic branch, relative to the strains isolated in Yunnan Province, China, which points to their common origin. Identified was a marker SNP and developed the method of SNP-typing for 1.ORI2v strains from Vietnam.
About the Authors
L. M. Kukleva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
K. A. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Zh. V. Al’khova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
N. I. Romanov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
N. Yu. Nosov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
A. V. Fadeeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
A. N. Malakhaeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
N. A. Sharapova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Z. L. Devdariani
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Yu. A. Popov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
Russian Federation
46, Universitetskaya St., Saratov, 410005, Russian Federation
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Review
For citations:
Kukleva L.M., Nikiforov K.A., Al’khova Zh.V., Romanov N.I., Nosov N.Yu., Fadeeva A.V., Malakhaeva A.N., Sharapova N.A., Devdariani Z.L., Popov Yu.A., Eroshenko G.A. Molecular-Genetic and Phenotypic Peculiarities of Plague Agent Strains Isolated in Vietnam. Problems of Particularly Dangerous Infections. 2017;(4):45-49. (In Russ.) https://doi.org/10.21055/0370-1069-2017-4-45-49
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