Taxonomic Position of Entomoparasitic Nematodes Isolated from Fleas in Gorno-Altai High-Mountain Natural Plague Focus
https://doi.org/10.21055/0370-1069-2018-2-79-83
Abstract
Objective of the study was to investigate entomoparasitic nematodes from rodent fleas, collected in Gorno-Altai high-mountain natural plague focus in 2016–2017, with identification of their taxonomic status. Materials and methods. Given are the results of morphological and genetic analysis of entomoparasitic nematodes – parasites of fleas Amphipsylla primaris primaris living on flat-headed high-mountain voles Alticola strelzovi, fleas Rhadinopsylla li transbaicalica of Daurian pika Ochotona dauurica, fleas Rhadinopsylla dahurica, collected at the burrows of the rodents. Results and conclusions. Identified have been nucleotide sequences of ITS2 spacer region of ribosomal operon in entomoparasitic nematodes of three flea species: Amphipsylla primaris primaris, Rhadinopsylla li transbaicalica, and Rhadinopsylla dahurica. Based on comparative phylogenetic analysis and identified high percentage of homology of the stated nucleotide sequences (92–99 %), appurtenance to Rubzovinema spp. species and close relation to earlier proposed by us multi-host species Rubzovinema polyxenica from C. tesquorum, A. rossica, and C. secundus fleas from Volga-Ural steppe plague focus has been established. For the first time ever separate branch of evolution of entomoparasitic nematodes – flea parasites, represented by Rubzovinema ssp., has been identified. Determined has been wide spread of Rubzovinema ssp. nematodes in biocenoses of steppe zone of Russia across Precaspian territory and Altai Mountains.
About the Authors
E. G. Oglodin
Russian Research Anti-Plague Institute “Microbe”, Saratov
Russian Federation
Russian Federation
E. G. Tokmakova
Irkutsk Research Anti-Plague Institute of Siberia and Far East, Irkutsk
Russian Federation
Russian Federation
K. A. Nikiforov
Russian Research Anti-Plague Institute “Microbe”, Saratov
Russian Federation
Russian Federation
A. V. Denisov
Altai Plague Control station, Gorno-Altaisk
Russian Federation
Russian Federation
N. A. Sharapova
Russian Research Anti-Plague Institute “Microbe”, Saratov
Russian Federation
Russian Federation
G. A. Eroshenko
Russian Research Anti-Plague Institute “Microbe”, Saratov
Russian Federation
Russian Federation
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Review
For citations:
Oglodin E.G., Tokmakova E.G., Nikiforov K.A., Denisov A.V., Sharapova N.A., Eroshenko G.A. Taxonomic Position of Entomoparasitic Nematodes Isolated from Fleas in Gorno-Altai High-Mountain Natural Plague Focus. Problems of Particularly Dangerous Infections. 2018;(2):79-83. (In Russ.) https://doi.org/10.21055/0370-1069-2018-2-79-83
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