Obtaining of Polyclonal Antibodies against B. anthracis S-layer Proteins and Study of their Specificity in Enzyme Immunoassay
https://doi.org/10.21055/0370-1069-2010-3(105)-46-49
Abstract
Purified preparations of B. anthracis S-layer proteins - Sap and EA1 were obtained. Developed was the scheme of laboratory animals` immunization, that enabled to receive rabbits' polyclonal antibodies against Sap and EA1 proteins in high titer. 12 strains of B. anthracis, 53 strains of closely-related bacilli and 4 strains of other heterogeneous bacteria were tested in enzyme-linked immunosorbent assay and DOT-immunoassay with the use of polyclonal anti-Sap and anti-EA1 antibodies. Analysis of specificity of the obtained antibodies revealed cross-reactions with B. cereus, B. subtilis, B. mycoides and B. megaterium. The specificity of polyclonal anti-EA1 and anti-Sap antibodies was 75 and 83 % respectively. Sensitivity of reaction was 105 and106 m.c. in applying polyclonal antibodies against B. anthracis S-layer proteins
Keywords
616.981.51:576.809.7
About the Authors
A. Yu. Goncharova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. I. Mikshis
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
O. M. Kudryavtseva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
M. F. Bolotnikova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
L. V. Novikova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
G. G. Krasichkov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
T. V. Alenkina
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
Yu. A. Popov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
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Review
For citations:
Goncharova A.Yu., Mikshis N.I., Kudryavtseva O.M., Bolotnikova M.F., Novikova L.V., Krasichkov G.G., Alenkina T.V., Popov Yu.A. Obtaining of Polyclonal Antibodies against B. anthracis S-layer Proteins and Study of their Specificity in Enzyme Immunoassay. Problems of Particularly Dangerous Infections. 2010;(3(105)):46-49. (In Russ.) https://doi.org/10.21055/0370-1069-2010-3(105)-46-49
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