Obtaining and Immunologic Characterization of Filoviruses Recombinant Proteins VP40 and NP
https://doi.org/10.21055/0370-1069-2010-4(106)-32-36
Abstract
Full-size genes coding matrix proteins VP40 of Ebola virus (VE) and Marburg virus (VM) and nucleoprotein (NP) of VE were cloned. These genes expression in Escherichia coli and purification of their products by affinity chromatography using Ni-NTA agarose permitted to obtain full-size recombinant VP40 and NP proteins of filoviruses. Immunological studies showed the recombinant polypeptides to possess antigenic properties similar to those of the native viral proteins. These proteins can be used for constructing of experimental vaccines against filoviruses and improving immunodiagnostic tests against filoviral infections.
About the Authors
A. V. Ivanova
State Scientific Center for Virusology and Biotechnology
Russian Federation
Russian Federation
E. I. Kazachinskaya
State Scientific Center for Virusology and Biotechnology
Russian Federation
Russian Federation
A. V. Kachko
State Scientific Center for Virusology and Biotechnology
Russian Federation
Russian Federation
E. L. Subbotina
State Scientific Center for Virusology and Biotechnology
Russian Federation
Russian Federation
A. V. Sorokin
State Scientific Center for Virusology and Biotechnology
Russian Federation
Russian Federation
I. A. Razumov
State Scientific Center for Virusology and Biotechnology
Russian Federation
Russian Federation
S. V. Neteosov
Novosibirsk State University
Russian Federation
Russian Federation
V. B. Loktev
State Scientific Center for Virusology and Biotechnology
Russian Federation
Russian Federation
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Review
For citations:
Ivanova A.V., Kazachinskaya E.I., Kachko A.V., Subbotina E.L., Sorokin A.V., Razumov I.A., Neteosov S.V., Loktev V.B. Obtaining and Immunologic Characterization of Filoviruses Recombinant Proteins VP40 and NP. Problems of Particularly Dangerous Infections. 2010;(4(106)):32-36. (In Russ.) https://doi.org/10.21055/0370-1069-2010-4(106)-32-36
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