Influence of Tularemia Microbe of Different Subspecies on the Functional Activity of Immunocompetent Cells of Experimental Animals

Analysis of molecular-biological mechanisms of patho-, immuno- and morphogenesis allowed to determine the role of bactericidal mechanisms (oxygen-, nitroxide-related and oxygen-free) of tularemia microbe phagocytosis by immunophagocytic system cells. Using PCR with primers pdpD-1F, pdpD-1R, pdpA 1, pdpA2 it was shown that the structure of pathogenicity islands (FPI) in F. tularensis strains of different subspecies depended upon what subspecies the strain belonged to, and was probably associated with tularemia microbe virulence. The macroorganism reaction to the introduction of tularemia microbe of different subspecies was evaluated. The obtained data were of prognostic significance for characterization of infectious process course and for the development of criteria for lymphoid organs response in pathologic and adaptive processes.

Francisella tularensis, macrophages, polymorphonuclear leukocytes, lymphocytes, cytokines, bacterial systems of phagocytes, immunocompetent organs

1. Дубровина В.И., Коновалова Ж.А., Колесникова О.Б., Татарников С.А., Витязева С.А., Войткова В.В. и др. Определение функционального состояния фагоцитов в качестве показателя неспецифической защиты организма (методические рекомендации). Иркутск; 2008. 9 с.

2. Дубровина В.И., Татарников С.А., Коновалова Ж.А., Мазепа А.В., Витязева С.А., Бельков А.И. и др. Функционально-метаболическая активность фагоцитов при взаимодействии с туляремийным микробом разных подвидов. Мед. паразитол. и паразитарн. бол. 2008; 2:17-21.

3. Лили Р. Патологическая техника и практическая гистохимия. М.: Мир; 1969. 645 с.

4. Broekhuijsen M., Larsson P., Johansson A., Bystrom M., Eriksson U., Larsson E. et al. Genome-wide DNA microarray analisis of Francisella tularensis strains demonstrates extensive genetic conservation within the highly virulent F. tularensis subsp. tularensis. J. Clin. Microbiol. 2003; 41(7):2924-31.

5. Green L.C., Wagner D A., Glogowski J., Skipper P.L., Wishnok J.S., Tannenbaum S.R. Analysis of nitrate, nitrite and [15N] in biological fluids. Anal. Bioch. 1982; 126(1):131-8.

6. Nano F.E., Zhang N., Cowley S.C., Klose K.E., Cheung K.M., Roberts M.J.. et al. A Francisella tularensis pathogenicity island required for intramacrophage growth. J. Bacteriol. 2004; 186(19):6430-36.