Development of Methodic Approach to Construction of Recombinant Strain, Producer of the Cholera Toxin B Subunit
https://doi.org/10.21055/0370-1069-2010-4(106)-62-65
Abstract
Presented are the results of experiments on the construction of Escherichia coli Top10 (pTrcSuB) strain, perspective for the development of recombinant cholera vaccine for peroral use. The obtained strain possesses inducible expression of cholera toxin B subunit. Peculiarities of cloned gene expression in strain-producer and the conditions of its maintaining were studied. The proposed approach will allow to obtain effective and safe cholera vaccines.
Keywords
616.932:615.371/.372
About the Authors
D. S. Yanov
48 Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
S. N. Klinova
48 Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
A. V. Mironin
48 Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
I. V. Zhivov
48 Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
I. P. Pogorelsky
48 Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
V. I. Drobkov
48 Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
Yu. N. Feodorov
48 Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
Ya. A. Kibirev
48 Central Research Institute of the Ministry of Defense of the Russian Federation
Russian Federation
Russian Federation
References
1. Девис Р., Ботстайн Д., Рот, Дж. Генетика бактерий. М.: Мир; 1984. 176 с.
2. Медуницын Н.В. Вакцинология. М.: Триада-Х; 1999. 272 с.
3. Онищенко Г.Г., Кутырев В.В., редакторы. Лабораторная диагностика опасных инфекционных болезней. Практическое руководство. М.: Медицина; 2009. 472 с.
4. Покровский В.И., редактор. Холера в СССР в период VII пандемии. М.: Медицина; 2000. 472 с.
5. Смирнова Н.И., Кобкова И.М., Ливанова Л.Ф., Чеховская Г.В., Коннов Н.П., Лозовский Ю.В. Экспрессия гомологичных и гетерололгичных генов протективных антигенов в клетках штамма Escherichia coli М17, используемого для изготовления лечебно-прфилактического препарата колибактерина. Биотехнология. 2006; 4:13-22.
6. Тертон М., Бангхем Д.Р., Колкотт К.А. Новые методы иммуноанализа. М.: Мир; 1991. 116 с.
7. Choi J. H., Lee S.Y. Secretory and extracellular production of recombinant proteins using Escherichia coli. Appl. Microbiol. Biotechnol. 2004; 64(5):625-35.
8. Hase C.C., Thai L.S. Construction and сharacterization of recombinant Vibrio cholerae strains producing inactive cholera toxin analogs. Infect. Immun.1994; 62(8):3051-7.
9. Kalambaheti T., Chaisri U., Srimanote P., Pongponratn E., Chaicumpa W. Immunogenicity and protective role of three formulations of oral cholera vaccine. Vaccine.1998; 16(2-3):201-7 .
10. Laemmli U.K. Cleavage of structural proteins during the assembly of bacteriophage T4. Nature. 1970; 227(5259):680-5.
11. Spira W.M., Sack R.B., Froehlich J.L. Simple adult rabbit model for Vibrio cholerae and enterotoxigenic Escherichia coli diarrhea. Infect. Immun. 1981; 32(2):739-47.
Review
For citations:
Yanov D.S., Klinova S.N., Mironin A.V., Zhivov I.V., Pogorelsky I.P., Drobkov V.I., Feodorov Yu.N., Kibirev Ya.A. Development of Methodic Approach to Construction of Recombinant Strain, Producer of the Cholera Toxin B Subunit. Problems of Particularly Dangerous Infections. 2010;(4(106)):62-65. (In Russ.) https://doi.org/10.21055/0370-1069-2010-4(106)-62-65
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