PCR Differentiation of Francisella tularensis Subspecies Using One Primer
https://doi.org/10.21055/0370-1069-2011-1(107)-46-48
Abstract
Presented are experimental data of size distribution of amplicons obtained with the help of the primer consisting of Escherichia coli chi-sequence and DNA from different subspecies of Francisella tularensis strains. Analysis of this distribution permitted to determine five informative regions on the electrophoregrams (in the regions of 190, 280, 500-570, 830 and 950 b.p.), that made it possible to perform subspecies differentiation of tularemia microbe strains. Thus, the subspecies of F. tularensis clinical isolates can be identified by safe, fast and convenient method - the PCR analysis using only one primer Chi 1f despite the slight genetic heterogeneity of tularemia agent strains.
About the Authors
G. M. Vakhrameeva
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. A. Lapin
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
V. M. Pavlov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. N. Mokrievich
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
R. I. Mironova
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
I. A. Dyatlov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
References
1. Olsuf’ev N.G. [Taxonomia, microbiology and laboratory diagnostics of tularemia agent]. Moscow: Medicina; 1975. 192 p.
2. Khesin N.B. [Genome variability]. Moscow: Nauka; 1984. 472 p.
3. Johansson A., Farlow J. Larsson P., Dukerich M., Chambers E., Bystrom M., Fox J., Chu M., Forsman M., Sjöstedt A., Keim P. Worldwide genetic relationships among Francisella tularensis isolates determined by multiple-locus variable-number tandem repeat analysis. J. Bacteriol. 2004; 186:5808-18.
4. Johansson A., Ibrahim A., Goransson I., Eriksson U., Gurycova D., Claridge III J.E., Sjostedt A. Evaluation of PCR-based methods for discrimination of Franciseella species and subspecies and development of a specific PCR that distinguishes two major subspecies of Francisella tularensis. J. Clin. Microbiol. 2000; 38(11):4180-5.
5. Kobayashi I., Murialdo H., Crasemann J.M., Stahl M.M., Stahl F.W. Orientation of cohesive end site cos determines the active orientation of χ sequence in stimulating recA-recBC-mediated recombination in phage λ lytic infections. Proc. Natl. Acad. Sci. USA. 1982; 79:5981-5.
6. Nutter J.E. Antigens of Pasteurella tularensis: preparative procedures. Appl. Microbiol. 1971; 22:44-8.
7. De la Puente-Redondo V.A., del Blanco N.G., Gutierrez-Martin C.B., Garcia-Pena F.J., Ferri E.F.R. Comparison of different Approaches for typing of Francisella tularensis strains. J. Clin. Microbiol. 2000; 38(3):1016-22.
8. Sambrook J., Fritch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. NY: Cold Spring Harbor Laboratory; 1989.
Review
For citations:
Vakhrameeva G.M., Lapin A.A., Pavlov V.M., Mokrievich A.N., Mironova R.I., Dyatlov I.A. PCR Differentiation of Francisella tularensis Subspecies Using One Primer. Problems of Particularly Dangerous Infections. 2011;(1(107)):46-48. (In Russ.) https://doi.org/10.21055/0370-1069-2011-1(107)-46-48
Views:
552
Email this article 