Altered Variants of Cholera Agent Isolated in the Territory of the Russian Federation
https://doi.org/10.21055/0370-1069-2011-1(107)-49-52
Abstract
Carried out was the retrospective genetic analysis of cholera vibrio strains which caused the outbreaks and sporadic cases of cholera in the territory of the Russian Federation from 1993 to 2006. It was elucidated that beginning from 1993 the disease had been caused by the altered strains of V. cholerae El Tor containing ctxB gene of classical type, encoding B subunit of cholera toxin, in their CTXφ prophage genome. In addition, identified were two strains whose prophage genome contained classical type rstR gene.
About the Authors
A. A. Goryaev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
S. P. Zadnova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
A. V. Shubina
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
Ya. M. Krasnov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. I. Smirnova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
References
1. Smirnova N.I., Kutyrev V.V. [Evolution of the cholera agent genome]. Mol. Gen. Mikrobiol. Virusol. 2004; 4:3–13.
2. Bhattacharya T., Chatterjee S., Maiti D., et al. Molecular analysis of the rstR and orfU genes of the CTX prophages integrated in the small chromosomes of environmental Vibrio cholerae non-O1, non-O139 strains. Environ. Microbiol. 2006; 8:526-34.
3. Chun J, Grim C.J., Hasan N.A., Lee J.H., Choi S.Y., Haley B.J., et al. Comparative genomics reveals mechanism for short-term and long-term clonal transitions in pandemic Vibrio cholerae. Proc. Natl. Acad. Sci. USA. 2009; 106(36):15442-7.
4. Faruque S.M., Nair G.B. Molecular ecology of toxigenic Vibrio cholerae. Microbiol. Immunol. 2002; 46:59-66.
5. Fields P.I., Popovic T., Wachsmuth K., Olsvik O. Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J. Clin. Microbiol. 1992; 30(8):2118-21.
6. Keasler S.P., Hall R.H. Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction. Lancet. 1993; 341(8861):1661.
7. Kumar P., Jain M., Goel A.K., Bhadauria S., Sharma S.K., Kamboj D.V., et al. A large cholera outbreak due to a new cholera toxin variant of the Vibrio cholerae O1 El Tor biotype in Orissa, Eastern India. J. Med. Microbiol. 2009; 58:234-8.
8. Morita M., Ohnishi M., Arakawa E., Bhuiyan N.A., Nusrin S., Alam M., et al. Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor. Microbiol. Immunol. 2008; 52:314-7.
9. Nair G.B., Faruque S.M., Bhuiyan N.A., Kamruzzaman M., Siddique A.K., Sack D.A. New variants of Vibrio cholerae O1 biotype EL Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J. Clin. Microbiol. 2002; 40:3296-9.
10. Safa A., Sultana J., Cam P.D., et al. Classical cholera toxin producing Vibrio cholerae O1 hybrid El Tor strains in Asia and Africa. Emerg. Infect. Dis. 2008; 14:987-8.
11. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24(8):1596-9.
12. Waldor M.K., Rubin E.J., Gregory D.N. et al. Regulation, replication, and integration functions of the Vibrio cholerae CTXφ are encoded by regions RS2. Mol. Microbiol. 1997; 24:917-26.
Review
For citations:
Goryaev A.A., Zadnova S.P., Shubina A.V., Krasnov Ya.M., Smirnova N.I. Altered Variants of Cholera Agent Isolated in the Territory of the Russian Federation. Problems of Particularly Dangerous Infections. 2011;(1(107)):49-52. (In Russ.) https://doi.org/10.21055/0370-1069-2011-1(107)-49-52
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