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Elaboration of the Algorithm of MLST-Typing of Pandemic and Pre-Pandemic V. cholerae El Tor strains

https://doi.org/10.21055/0370-1069-2011-1(107)-58-61

Abstract

Molecular typing of pandemic and pre-pandemic cholera vibrio strains was carried out with the help of multi-locus sequence analysis. Application of two MLST schemes based on the sequence of virulence-associated and housekeeping genes demonstrated the last one to be the most effective in differentiation of strains, isolated before and during the seventh cholera pandemic.

About the Authors

A. V. Ossin
Russian Research Anti-Plague Institute Microbe
Russian Federation


Ya. M. Krasnov
Russian Research Anti-Plague Institute Microbe
Russian Federation


N. P. Guseva
Russian Research Anti-Plague Institute Microbe
Russian Federation


N. I. Smirnova
Russian Research Anti-Plague Institute Microbe
Russian Federation


References

1. Smirnova N.I., Nefedov K.S., Osin A.V., Livanova L.F., Krasnov Ia.M. [A Study of the Prevalence of Regulatory Genes Controlling Virulence Gene Expression among Vibrio cholerae Eltor Strains Varying in Their Pandemic Potential]. Mol. Gen. Mikrobiol. Virusol. 2007; 1:15–22.

2. Balakrish Nair, Ashrafus Safa, Bhuiyan N. A., Suraia Nusrin, Denise Murphy, Carolyn Nicol et al. Isolation of Vibrio cholerae O1 strains similar to pre-seventh pandemic El Tor strains during an outbreak of gastrointestinal disease in an island resort in Fiji. J. Med. Microbiol. 2006; 55:1559-62.

3. Dziejman M., Balon E., Boyd D., Fraser C.M., Heidelberg J.F., Mekalanos J.J. Comparative genomic analysis of Vibrio cholerae: genes that correlate with cholera endemic and pandemic disease. Proc. Natl. Acad. Sci. USA. 2002; 99(3):1556-61.

4. Garg P., Aydanian A., Smith D., Morris J.G., Balakrish Nair G., Stine O.C. Molecular epidemiology of O139 Vibrio cholerae: mutation, lateral gene transfer, and founder flush. Emer. Infec. Diseases. 2003; 9(7):810-4.

5. Grim C.J., Hasan N.A., Taviani E., Haley B., Chun J., Brettin T.S. et al. Genome sequence of hybrid Vibrio cholerae O1 MJ-1236, B-33, and CIRS101 and comparative genomics with V. cholerae. J. Bacteriol. 2010; 192(13):3524-33.

6. MLST-Home: Multi Locus Sequence Typing [Internet] Available from: http://www.mlst.net [cited 17 Jun 2010].

7. Jolley K.A., Feil E.J., Chan M.S., Maiden M.C. Sequence type analysis and recombinational tests (START). Bioinformatics. 2001; 17:1230-1.

8. Kumar S., Tamura K., Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics. 2004; 5:150-63.

9. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors Proc. Natl. Acad. Sci. USA. 1977; 74:5463-7.


Review

For citations:


Ossin A.V., Krasnov Ya.M., Guseva N.P., Smirnova N.I. Elaboration of the Algorithm of MLST-Typing of Pandemic and Pre-Pandemic V. cholerae El Tor strains. Problems of Particularly Dangerous Infections. 2011;(1(107)):58-61. (In Russ.) https://doi.org/10.21055/0370-1069-2011-1(107)-58-61

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ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)