Phenotypic and Molecular-Genetic Peculiarities of Yersinia pestis Strains from Trans-Baikal Steppe Plague Focus

The paper contains the data on the complex phenotypic and genotypic characteristics of
Yersinia pestisstrains isolated in Trans-Baikal steppe plague focus before and after the succession of the main carrier which took place in the 1960s. Based on the PCR-analysis and multilocus sequence typing of
glpD, melB, napA, rhaA, and
iclR genes, encoding differentially significant properties such as glycerin, melibiose, rhamnose fermentation, isocitrate lyase production, and denitrification activity, developed are the proofs of affiliation of the studied strains to the biovariant antiqua of the plague agent main subspecies. Multilocus VNTR-analysis by seven locuses of variable tandem repeats – ms01, ms04, ms06, ms07, ms46, ms62 – has been used to establish genetic relations between the strains. Thus on the basis of the results obtained, it is established that the strains of the plague agent, isolated in the Trans-Baikal steppe focus in various periods of its existence, form one common branch on the phylogenetic tree of
Y. pestis evolution.

возбудитель чумы, природные очаги, биовары, фенотипическая и генетическая характеристика штаммов, генотипирование, plague agent, natural foci, biovars, phenotypic and genotypic characteristics of strains, genotyping

1. Danilenko A.F., Nemchenko L.S. [Current state of the Trans-Baikal natural plague focus at the stage of sanitation enhancement]. In: [Topical Issues of Prophylaxis of Particularly Dangerous and Natural-Focal Infectious Diseases]. Irkutsk; 1994. P. 39–40.

2. Eroshenko G.A., Odinokov G.N., Kukleva L.M., Shavina N.Yu., Kutyrev V.V. [Structural analysis of the genes participating in the process of melibiose fermentation and isocitrate lyase production in Yersinia pestis strains of the main and non-main subspecies]. Genetika. 2011; 47(10):328–34.

3. Eroshenko G.A., Odinokov G.N., Kukleva L.M., Pavlova A.I., Krasnov Ya.M., Shavina N.Yu., Guseva N.P., Vinogradova N.A., Kutyrev V.V. [Standardized algorithm of molecular typing of Yersinia pestis strains]. Zh. Epidemiol. Mikrobiol. Immunobiol. 2012; 3:25–35.

4. Koukleva L.M., Yeroshenko G.A., Kouklev V.E., Krasnov Ya.M., Guseva N.P., Odinokov G.N., Kutyrev V.V. [Comparison of complete nucleotide sequence of rhaS gene in the strains of plague etiological agent of main and minor subspecies]. Probl. Osobo Opasn. Infek. 2008; (97):38–42.

5. Odinokov G.N., Eroshenko G.A., Vidyaeva N.A., Krasnov Ja.M., Gouseva N.P., Kutyrev V.V. [Structural and functional analysis of nap operon genes in Yersinia pestis strains of different subspecies]. Probl. Osobo Opasn. Infek. 2008; (98):40–2.

6. Odinokov G.N., Eroshenko G.A., Krasnov Ya.M., Koukleva L.M., Shavina N.Yu., Pavlova A.I., Kutyrev V.V. [Genetic background of methionine dependency in Yersinia pestis strains of the main and non-main subspecies]. Genetika. 2011; 47(3):332–8.

7. Onishchenko G.G., Kutyrev V.V., editors [Laboratory Diagnostics of Particularly Dangerous Infections. Practice Guidelines]. M.: Meditsina; 2009. 472 p.

8. Onishchenko G.G., KutyrevV.V., editors [Natural Plague Foci in the Territory of the Caucasus, Caspian sea Region, Central Asia, and Siberia]. M.: Meditsina; 2004. 192 p.

9. [Activity management of the laboratories that employ methods of nucleic acid amplification for work with materials containing microorganisms of the I–IV groups of pathogenicity]. MR 1.3.2569-09. M.; 2009. 42 p.

10. Pavlova A.I., Eroshenko G.A., Odinokov G.N., Koukleva L.M., Shavina N.Yu., Krasnov Ya.M., Kutyrev V.V. [Analysis of genetic variability of Yersinia pestis strains (Medieval biovar) isolated in natural plague foci of the Russian Federation and Mongolia]. Probl. Osobo Opasn. Infek. 2012; (114):49–53.

11. Popov N.V., Bezsmertny V.E., Matrosov A.N., Nemchenko L.S., Verzhutsky D.B., Maletskaya O.V., Udovikov A.I., Kuznetsov A.A., Knyazeva T.V., Shilova L.D., Gorshenko V.V., Popov V.P., Toporkov V.P., Toporkov A.V., Kutyrev V.V. [Epizootic activity of plague natural foci in the Russian Federation in 2010 and prognosis for 2011]. Probl. Osobo Opasn. Infek. 2011; (107):31–7.

12. Suchkov I.Yu., Vodop’yanov A.S., Vodop’yanov S.O., Shishiyanu M.V., Mishan’kin B.N. [Multi-locus VNTR-analysis for investigations of Yersinia pestis population structure in natural foci]. Mol. Genet. Mikrobiol. Virusol. 2004; 4:19–28.

13. Le Flèche P., Hauck Y., Onteniente L., Prieur A., Denoeud F., Ramisse V., Sylvestre P., Benson G., Ramisse F., Vergnaud G. A tandem repeats database for bacterial genomes: application to the genotyping of Yersinia pestis and Bacillus anthracis. BMC Microbiol. 2001; 1:2.

14. Motin V., Georgescu A., Elliott J., Hu P., Worsham P., Ott L., Slezak T., Sokhansanj B., Regala W., Brubaker R., Garcia E. Genetic variability of Yersinia pestis isolates as predicted by PCR-based IS100 genotyping and analysis of structural genes encoding glycerol-3-phosphate dehydrogenase (glpD). J. Bacteriol. 2002; 184(4):1019–27.