Obtainment of the Immunomagnetic Particles for Francisella tularensis Cells Detection
https://doi.org/10.21055/0370-1069-2011-3(109)-50-53
Abstract
Obtained are the immunomagnetic particles with the immobilized highly-specific monoclonal antibodies FB 11-x to Francisella tularensis. Their usage in enzyme-linked immunoassays made it possible to detect 105 - 106 CFU/ml of F. tularensis. The binding of the bacterial cells with IMP was confirmed by the results of fluorescent microscopy.
Keywords
Francisella tularensis,
immunomagnetic particles,
monoclonal antibodies,
enzyme-linked immunoassay,
fluorescent microscopy
616.981.455:616-097
About the Authors
S. S. Vetchinin
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
P. Kh. Kopylov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
N. V. Kiseleva
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
O. G. Nikolaeva
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. V. Gavryushkin
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
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Review
For citations:
Vetchinin S.S., Kopylov P.Kh., Kiseleva N.V., Nikolaeva O.G., Gavryushkin A.V. Obtainment of the Immunomagnetic Particles for Francisella tularensis Cells Detection. Problems of Particularly Dangerous Infections. 2011;(3(109)):50-53. (In Russ.) https://doi.org/10.21055/0370-1069-2011-3(109)-50-53
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