Peculiarities of the Effect of the Lipopolysaccharide of Tularemia Microbe of Different Subspecies upon Metabolic Activity of Phagocytes in Vitro
https://doi.org/10.21055/0370-1069-2011-2(108)-57-60
Abstract
Experimental preparations of the lipopolysaccharide (LPS) of Francisella tularensis of four subspecies (tularensis, holarctica, mediasiatica, novicida) were obtained using Tween extraction method. Physical and chemical characteristics of the obtained preparations are presented. Examined was the effect of tularemia agent of different subspecies upon the phagocytic part of the experimental animals` immunity in vitro. LPS biomolecules demonstrated weak stimulating effect upon oxygen- and nitroxide-dependent bactericidal systems and lacked cytopathogenicity for phagocytes apoptosis activation. The obtained results suggest that such inactivity of tularemia agent LPS is associated with its structural peculiarities.
About the Authors
V. I. Dubrovina
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
Zh. A. Konovalova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
V. B. Nikolaev
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
V. V. Voitkova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
S. A. Tatarnikov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
A. V. Mazepa
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
E. Yu. Markov
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
Yu. O. Popova
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
O. V. Yur`eva
Irkutsk Research Anti-Plague Institute of Siberia and Far East
Russian Federation
Russian Federation
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Review
For citations:
Dubrovina V.I., Konovalova Zh.A., Nikolaev V.B., Voitkova V.V., Tatarnikov S.A., Mazepa A.V., Markov E.Yu., Popova Yu.O., Yur`eva O.V. Peculiarities of the Effect of the Lipopolysaccharide of Tularemia Microbe of Different Subspecies upon Metabolic Activity of Phagocytes in Vitro. Problems of Particularly Dangerous Infections. 2011;(2(108)):57-60. (In Russ.) https://doi.org/10.21055/0370-1069-2011-2(108)-57-60
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