Development of VNTR-Based Test System for V. cholerae Typing
https://doi.org/10.21055/0370-1069-2011-2(108)-61-63
Abstract
Analysis of VNTRs was used for typing of V. cholerae strains deposited in microbial culture collection of 48 Central Research Institute. The most informative loci and flanking primers were chosen, VNTR profiles were compiled, and allele variability analysis of the strains was carried out. Laboratory series of test system were manufactured . The developed test system can be used for epidemiological monitoring and investigations, as well as for passportization of collection strains.
About the Authors
Ya. A. Kibirev
Russian Federation Ministry of Defense 48 Central Research Institute
Russian Federation
Russian Federation
I. V. Darmov
Russian Federation Ministry of Defense 48 Central Research Institute
Russian Federation
Russian Federation
M. A. Loginova
Russian Federation Ministry of Defense 48 Central Research Institute
Russian Federation
Russian Federation
I. V. Marakulin
Russian Federation Ministry of Defense 48 Central Research Institute
Russian Federation
Russian Federation
D. A. Chukhlantsev
Russian Federation Ministry of Defense 48 Central Research Institute
Russian Federation
Russian Federation
T. I. Dormidontova
Russian Federation Ministry of Defense 48 Central Research Institute
Russian Federation
Russian Federation
S. L. Kuznetsov
Russian Federation Ministry of Defense 48 Central Research Institute
Russian Federation
Russian Federation
A. V. Kuznetsovskii
Russian Federation Ministry of Defense 48 Central Research Institute
Russian Federation
Russian Federation
References
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3. The Microorganisms Tandem Repeats Database. Available from: http://minisatellites.u-psud.fr/ASPSamp/base_ms/bact.php.
4. Kaper, J.B., Morris J.G., Levine M.M. Cholera. Clin. Microbiol. Rev. 1995; 8:48-86.
Review
For citations:
Kibirev Ya.A., Darmov I.V., Loginova M.A., Marakulin I.V., Chukhlantsev D.A., Dormidontova T.I., Kuznetsov S.L., Kuznetsovskii A.V. Development of VNTR-Based Test System for V. cholerae Typing. Problems of Particularly Dangerous Infections. 2011;(2(108)):61-63. (In Russ.) https://doi.org/10.21055/0370-1069-2011-2(108)-61-63
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