Study of Biological Effect of CHO-Cell Elongating Factor of Vibrio cholerae on Models in vitro and in vivo
https://doi.org/10.21055/0370-1069-2012-1(111)-62-65
Abstract
Studied were morphological, physiological and ultrastructural changes in the cell culture L-929 and in the intestine of suckling mice in the presence of Cef (CHO cell elongating factor) of Vibrio cholerae. Registered were cellular elongation, cytoplasm vacuolization, an increase in the number of lysosomes, retraction and nuclei deformation in the L-929 cell culture. Statistical significance test confirmed that in vivo Cef caused the accumulation of fluid in suckling mice. Electron microscopy of the epithelial cells of small intestine revealed vacuolization and myelin-like structures formation in cytoplasm and nuclei, swelling of mitochondria, clarification of their matrix and destruction of cristae. Cristae were filled with mucus. The involvement of mast cells caused the increasing of capillary permeability which enhanced dehydration. These changes confirmed Cef participation in manifestation of Vibrio cholerae pathogenic properties.
Keywords
616.932
About the Authors
E. V. Monakhova
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
G. M. Fedorenko
Southern Scientific Centre of Russian Academy of Sciences
Russian Federation
Russian Federation
A. B. Mazrukho
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
R. V. Pisanov
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
V. D. Kruglikov
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
O. V. Markina
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
L. P. Alekseeva
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
References
1. VlasovV.P., Kharlanova N.G., Monakhova E.V., Vinokur N.I., Bardakhch’yan E.A. [Ultrasructural changes in the large intestine, caused by choleragenic and non-choleragenic vibrio strains]. Doklady AN. 1997; 352:278–80.
2. Monakhova E.V., Lomov Yu. M., Pisanov R.V., Alekseeva L.P., Markina O.V., Mironova A.V. et al. [Cloning of the gene of Vibrio cholerae CHO-cell elongating factor and its expression under control of PBAD promot- er]. Biotekhnologia. 2006; 5:12–8.
3. Monakhova E.V., Lomov Yu. M., Pisanov R.V., Alekseeva L.P., Markina O.V., Verkina L.M., Mironova A.V. [ Recombinant plasmid, express- ing the cloned cef (CHO cell elongating factor) of Vibrio cholerae (variants) and Escherichia coli strain – producer of cef (CHO cell elongating factor) of Vibrio cholerae (variants)]. RF Patent № 2313577.
4. McCardell B.A., Kothary M.H., Hall R.N., Sathyamoorthy V. Identification of a CHO-elongating factor produced by Vibrio cholerae O1. Microb. Pathogen. 2000; 29(1):1-8.
5. McCardell B.A., Sathyamoorthy V., Michalski J., Lavu S., Kothary M., Livezey J. et al. Cloning, expression and characterization of the CHO cell elongating factor (Cef) from Vibrio cholerae O1. Microb. Pathog. 2002; 32:165-72.
6. Sathyamoorthy V., Hall R.H., McCardell B.A., Kothary M., Ahn S.J., Ratnayake S. Purification and characterization of a cytotonic protein expressed in vitro by the live cholera vaccine candidate CVD 103-HgR. Infect. Immun. 2000; 68:6062-5.
Review
For citations:
Monakhova E.V., Fedorenko G.M., Mazrukho A.B., Pisanov R.V., Kruglikov V.D., Markina O.V., Alekseeva L.P. Study of Biological Effect of CHO-Cell Elongating Factor of Vibrio cholerae on Models in vitro and in vivo. Problems of Particularly Dangerous Infections. 2012;(1(111)):62-65. (In Russ.) https://doi.org/10.21055/0370-1069-2012-1(111)-62-65
Views:
592
Email this article 